| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L; MGC34403 |
| Entrez GeneID | 6500 |
| clone | 4E11 |
| WB Predicted band size | 19kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SKP1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于SKP1抗体的3篇参考文献及其摘要概括：
1. **《SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box》**
*作者：Bai, C., Sen, P., Hofmann, K., et al.*
摘要：该研究首次揭示了SKP1作为SCF泛素连接酶复合体的核心组分，通过F-box结构域与细胞周期调控蛋白(如Cyclin依赖性激酶抑制剂)结合，介导其泛素化降解，从而调控细胞周期进程。文中还探讨了SKP1抗体的应用，用于检测SCF复合体的组装及功能。
2. **《Role of SKP1 in colorectal cancer progression and its clinical significance》**
*作者：Wang, Y., Zhang, L., Li, J., et al.*
摘要：研究通过免疫组化(IHC)和Western blot分析，发现SKP1在结直肠癌组织中高表达，并与患者预后不良相关。利用特异性SKP1抗体证实其通过调控β-catenin稳定性促进肿瘤侵袭，提示SKP1可能作为结直肠癌治疗的潜在靶点。
3. **《Development and validation of a monoclonal antibody specific for human SKP1 protein》**
*作者：Chen, H., Xu, X., Luo, Q., et al.*
摘要：本研究报道了一种高特异性抗人SKP1单克隆抗体的开发与验证。通过ELISA、免疫沉淀(IP)及免疫荧光(IF)实验证明该抗体可识别内源性SKP1.并成功应用于多种肿瘤细胞系中SKP1蛋白定位及表达水平的检测，为相关机制研究提供了工具。
4. **《SKP1 modulates HIF-1α ubiquitination under hypoxic conditions》**
*作者：Kim, M., Lee, S., Park, J., et al.*
摘要：文章阐明了SKP1在低氧条件下通过调控HIF-1α的泛素化降解影响肿瘤代谢适应。研究使用SKP1抗体进行Co-IP实验，证明其与VHL蛋白的相互作用，并揭示了SKP1在肿瘤微环境中的新功能。
注：以上文献信息为基于领域知识的合理虚构示例，实际引用时需以真实文献为准。
**Background of SKP1 Antibody**
SKP1 (S-phase kinase-associated protein 1) is a core component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex, which plays a critical role in the ubiquitin-proteasome system by targeting specific proteins for degradation. This process regulates diverse cellular functions, including cell cycle progression, signal transduction, and DNA repair. SKP1 acts as an adaptor protein, bridging F-box proteins (substrate receptors) and Cullin-1 to enable substrate recognition and polyubiquitination. Dysregulation of SKP1 or SCF complexes is implicated in cancers, neurodegenerative diseases, and developmental disorders.
SKP1 antibodies are essential tools for studying the expression, localization, and interaction partners of SKP1 in various biological contexts. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to explore SKP1's role in cellular homeostasis and disease mechanisms. Additionally, these antibodies aid in investigating post-translational modifications of SKP1 or its involvement in specific pathways, such as the ubiquitination of tumor suppressors (e.g., p27) or oncoproteins. Researchers also utilize SKP1 antibodies to assess the impact of SKP1 depletion or overexpression in model systems, providing insights into its therapeutic potential. Validated SKP1 antibodies ensure specificity and reliability, making them crucial for both basic research and drug discovery efforts.
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