| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | Q360QC |
| Uniprot No | P03023 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQC |
| 预测分子量 | 44.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于Q360QC重组蛋白相关研究的模拟参考文献(注：Q360QC重组蛋白的具体信息可能涉及领域或命名差异，建议结合实际研究背景进一步核实)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Q360QC Mutant Protein in Inflammatory Signaling*
**作者**：Zhang, L. et al.
**摘要**：本研究成功在大肠杆菌中表达了Q360QC突变体重组蛋白，并通过体外实验证实该突变导致其与TNF-α受体的结合能力显著降低，提示该位点可能参与炎症信号通路的调控。
2. **文献名称**：*Structural Analysis of Q360QC Variant Reveals Altered Conformation in ATP-Binding Domains*
**作者**：Kim, S.J. & Patel, R.
**摘要**：通过X射线晶体学解析了Q360QC突变蛋白的三维结构，发现第360位谷氨酰胺(Q)到半胱氨酸(C)的突变引起ATP结合口袋构象变化，可能影响其激酶活性。
3. **文献名称**：*Q360QC Recombinant Protein as a Novel Antigen for Autoantibody Detection in Autoimmune Diseases*
**作者**：Garcia, M. et al.
**摘要**：开发了基于Q360QC重组蛋白的ELISA检测方法，证实其在系统性红斑狼疮患者血清中特异性抗体检出率较野生型提高32%，具有潜在诊断价值。
4. **文献名称**：*Optimization of Q360QC Protein Purification and Its Application in Drug Screening Assays*
**作者**：Wang, Y. et al.
**摘要**：建立了一种高效纯化Q360QC重组蛋白的亲和层析方案，并用于高通量筛选靶向该蛋白的小分子抑制剂，成功鉴定出两种具有纳摩尔级抑制活性的先导化合物。
**提示**：以上内容为模拟生成，若需真实文献，建议通过PubMed、Google Scholar等平台结合具体研究背景(如疾病领域、蛋白功能)进一步检索。
**Background of Q360QC Recombinant Protein**
The Q360QC recombinant protein is an engineered variant designed to address specific functional or structural challenges in biomedical research or therapeutic applications. The designation "Q360QC" typically reflects a mutation at position 360 in the amino acid sequence, where a glutamine (Q) is substituted with a cysteine (C). This modification is often introduced to enhance protein stability, facilitate site-specific conjugation (e.g., for drug labeling or PEGylation), or alter binding properties.
Recombinant proteins like Q360QC are produced using biotechnological platforms such as *E. coli*, yeast, or mammalian cell systems. The choice of expression system depends on the protein’s complexity, post-translational modification requirements, and intended use. For instance, if the protein requires disulfide bond formation or glycosylation, mammalian cells (e.g., CHO or HEK293) might be prioritized.
The development of Q360QC may stem from efforts to improve pharmacokinetics, reduce immunogenicity, or enable targeted delivery in therapeutic contexts. Such proteins are frequently explored in oncology, immunology, or enzyme replacement therapies. For example, introducing a cysteine residue at position 360 could allow precise attachment of cytotoxic payloads in antibody-drug conjugates (ADCs) or improve half-life through controlled PEGylation strategies.
Characterization of Q360QC involves rigorous quality control, including SDS-PAGE, mass spectrometry, and functional assays to confirm structural integrity and activity. Its applications span drug development, diagnostic tools, and mechanistic studies, underscoring the versatility of recombinant protein engineering in advancing precision medicine and biopharmaceutical innovation.
In summary, Q360QC exemplifies how targeted amino acid substitutions can optimize recombinant proteins for enhanced functionality, stability, and therapeutic potential in diverse biomedical fields.
×
