| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Akr1c13 |
| Uniprot No | Q8VC28 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-323aa |
| 氨基酸序列 | MSSKQHCVKLNDGHLIPALGFGTYKPKEVPKSKSLEAACLALDVGYRHVDTAYAYQVEEEIGQAIQSKIKAGVVKREDLFITTKLWCTCFRPELVKPALEKSLKKLQLDYVDLYIMHYPVPMKSGDNDFPVNEQGKSLLDTVDFCDTWERLEECKDAGLVKSIGVSNFNHRQLERILNKPGLKYKPVCNQVECHLYLNQRKLLDYCESKDIVLVAYGALGTQRYKEWVDQNSPVLLNDPVLCDVAKKNKRSPALIALRYLIQRGIVPLAQSFKENEMRENLQVFGFQLSPEDMKTLDGLNKNFRYLPAEFLVDHPEYPFVEEY |
| 预测分子量 | 44.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Akr1c13重组蛋白的虚构参考文献示例(仅供示例，非真实文献)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant AKR1C13 in Steroid Metabolism"
**作者**: Zhang L, et al. (2022)
**期刊**: *Biochemistry and Molecular Biology Reports*
**摘要**: 本研究在大肠杆菌中成功表达并纯化了AKR1C13重组蛋白，证实其具有NADPH依赖的还原酶活性，可催化雄烯二酮(androstenedione)转化为睾酮，提示其在类固醇激素代谢中的潜在作用。
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2. **文献名称**: "Structural Insights into AKR1C13 Substrate Specificity via Crystallographic Analysis"
**作者**: Tanaka K, et al. (2021)
**期刊**: *Journal of Structural Biology*
**摘要**: 通过X射线晶体学解析AKR1C13重组蛋白的3D结构，揭示其底物结合口袋的关键氨基酸残基，解释了其对孕酮和前列腺素的选择性催化机制。
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3. **文献名称**: "AKR1C13 Knockout Mice Reveal Its Role in Bile Acid Homeostasis"
**作者**: Gupta R, et al. (2020)
**期刊**: *Molecular Endocrinology*
**摘要**: 利用AKR1C13重组蛋白进行体外酶活实验，结合基因敲除小鼠模型，发现该酶通过调节胆汁酸前体的还原反应影响肝脏代谢平衡。
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4. **文献名称**: "AKR1C13 as a Potential Biomarker in Prostate Cancer: Recombinant Protein-Based Screening"
**作者**: Wang Y, et al. (2019)
**期刊**: *Cancer Research Communications*
**摘要**: 基于AKR1C13重组蛋白的高通量抑制剂筛选实验，发现其活性在前列腺癌组织中显著上调，提示其可作为治疗靶点或诊断标志物。
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**说明**：以上文献为示例性质，实际研究需通过PubMed、Google Scholar或Web of Science等平台检索真实文献。
Akr1c13. a member of the aldo-keto reductase (AKR) superfamily, is an enzyme involved in the metabolism of endogenous and exogenous substrates, including steroids, prostaglandins, and xenobiotics. This cytosolic protein catalyzes NADPH-dependent reduction reactions, converting carbonyl-containing compounds into their corresponding alcohols. It shares structural and functional similarities with other AKR1C isoforms, characterized by a conserved (α/β)8 barrel fold and a cofactor-binding domain.
The AKR1C subfamily plays critical roles in hormone regulation, particularly in steroidogenesis and inactivation. While human AKR1C isoforms are well-studied in contexts like androgen and progesterone metabolism, Akr1c13 is primarily characterized in rodents. It demonstrates substrate specificity for dihydrotestosterone (DHT), progesterone, and prostaglandin D2 (PGD2), suggesting involvement in regulating local hormone levels and inflammatory responses. In mice, Akr1c13 is highly expressed in reproductive tissues, liver, and adrenal glands, implicating its role in endocrine functions.
Recombinant Akr1c13 protein is produced through heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its recombinant form enables detailed biochemical characterization, including kinetic parameters, inhibitor screening, and structural analysis via X-ray crystallography. Research focuses on its potential role in diseases linked to hormonal imbalances, such as prostate cancer, endometriosis, and metabolic disorders. Additionally, Akr1c13-mediated prostaglandin metabolism may influence pain signaling and inflammation pathways, making it a target for therapeutic intervention.
Despite progress, species-specific functional variations between rodent Akr1c13 and human homologs (e.g., AKR1C3) complicate translational research. Ongoing studies aim to clarify its physiological significance and explore its utility as a biomarker or drug target in steroid-related pathologies.
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