| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AK5 |
| Uniprot No | Q9Y6K8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-562aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMNTNDAKEYMARREIPQLFESLLNGL MCSKPEDPVEYLESCLQKVKELGGCDKVKWDTFVSQEKKTLPPLNGGQSR RSFLRNVMPGNSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFD PTRPRPKIILVIGGPGSGKGTQSLKIAERYGFQYISVGELLRKKIHSTSS NRKWSLIAKIITTGELAPQETTITEIKQKLMQIPDEEGIVIDGFPRDVAQ ALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLM NFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAA AGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKC KIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIR DIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRR IGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASI PVIAYYETKTQLHKINAEGTPEDVFLQLCTAIDSIF |
| 预测分子量 | 66 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与AK5重组蛋白相关的模拟参考文献(注：部分文献信息为示例性概括，实际研究中请以真实文献为准)：
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1. **文献名称**: "Structural and Functional Characterization of Recombinant Human Adenylate Kinase 5"
**作者**: Smith J. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人源AK5蛋白，通过X射线晶体学解析了其三维结构(分辨率2.1Å)，揭示了其底物结合口袋的独特构象，并验证了其催化AMP磷酸化为ADP的活性，为靶向AK5的药物设计提供了结构基础。
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2. **文献名称**: "AK5 Recombinant Protein Modulates Neuronal Energy Metabolism in Alzheimer's Disease Models"
**作者**: Zhang L. et al.
**摘要**: 利用哺乳动物细胞系统表达重组AK5蛋白，研究发现其可通过调控脑细胞ATP/ADP平衡缓解β-淀粉样蛋白诱导的神经元能量代谢障碍，提示AK5在阿尔茨海默病病理中的潜在治疗价值。
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3. **文献名称**: "Expression Optimization and Functional Analysis of AK5 in Colorectal Cancer Cells"
**作者**: Lee H. et al.
**摘要**: 通过密码子优化和低温诱导策略提高AK5重组蛋白在HEK293细胞中的表达量，并证实其在结直肠癌细胞中过表达可促进细胞增殖，可能与肿瘤能量代谢重编程相关。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“recombinant AK5 protein”或“adenylate kinase 5 expression”。
**Background of AK5 Recombinant Protein**
Adenylate kinase 5 (AK5), a member of the adenylate kinase family, is an enzyme critical for cellular energy metabolism and nucleotide homeostasis. It catalyzes the reversible transfer of phosphate groups between adenosine nucleotides (ATP, ADP, and AMP), maintaining energy equilibrium in cells. Unlike other adenylate kinases, AK5 exhibits tissue-specific expression, predominantly found in the brain, testis, and certain cancer cells, suggesting specialized roles in these tissues.
The recombinant AK5 protein is produced using biotechnological methods, typically via expression in bacterial (e.g., *E. coli*) or mammalian cell systems. The gene encoding AK5 is cloned into an expression vector, transfected into host cells, and induced to produce the protein. Post-expression, purification techniques such as affinity chromatography (e.g., His-tag systems) or ion-exchange chromatography ensure high purity and functionality.
Research on AK5 has gained momentum due to its potential implications in disease mechanisms. Studies link AK5 dysregulation to neurological disorders, cancer progression, and metabolic syndromes. For instance, reduced AK5 activity in the brain has been associated with neurodegenerative conditions, while its overexpression in tumors may support rapid proliferation by enhancing energy supply. Additionally, AK5’s role in nucleotide metabolism makes it a candidate for drug targeting, particularly in therapies aimed at disrupting cancer cell energetics.
As a recombinant tool, AK5 protein facilitates *in vitro* studies, including enzymatic assays, inhibitor screening, and structural analyses (e.g., X-ray crystallography). Its availability also aids in antibody production for diagnostic applications. Overall, AK5 recombinant protein serves as a vital resource for unraveling cellular energy dynamics and developing therapeutic strategies for related diseases.
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