| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MG281 |
| Uniprot No | P47523 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-556aa |
| 氨基酸序列 | MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVASGRSGNLGFKLFSTQSPSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDKWTYFAAKGSPLYDSYPNHFFEDVKTLAIDAKDISALKTTIDSEKPTYLIIRGLSGNGSQLNELQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL |
| 预测分子量 | 65.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MG281重组蛋白的模拟参考文献示例(注：以下为虚构内容，仅作格式参考)：
1. **文献名称**：*Structural characterization of recombinant MG281 protein from Mycoplasma genitalium*
**作者**：Smith A, et al.
**摘要**：本研究通过X射线晶体学解析了MG281重组蛋白的三维结构，揭示了其表面抗原结构域与宿主细胞受体互作的关键位点，为理解支原体黏附机制提供结构基础。
2. **文献名称**：*Recombinant MG281 protein as a novel serodiagnostic antigen for Mycoplasma genitalium infections*
**作者**：Zhang L, et al.
**摘要**：研究评估了重组MG281蛋白在ELISA检测中的性能，证实其对临床样本中支原体抗体具有高特异性，可作为新型血清学诊断标志物。
3. **文献名称**：*Immunomodulatory effects of MG281 recombinant protein in host-pathogen interaction*
**作者**：Tanaka K, et al.
**摘要**：实验发现MG281重组蛋白可通过抑制TLR4信号通路干扰宿主先天免疫应答，提示其在支原体免疫逃逸中的潜在作用机制。
4. **文献名称**：*Expression optimization and functional validation of MG281 recombinant protein in E. coli*
**作者**：Wang X, et al.
**摘要**：通过密码子优化和纯化工艺改进，实现了MG281蛋白的高效可溶性表达，并验证其体外促进支原体聚集的生物活性。
注：以上内容基于学术文献常见研究方向模拟创作，非真实存在的论文。实际文献检索建议通过PubMed或Web of Science等数据库进行。
**Background of MG281 Recombinant Protein**
MG281 is a recombinant protein derived from *Mycoplasma genitalium*, a pathogenic bacterium known for its role in sexually transmitted infections. As one of the smallest free-living organisms, *M. genitalium* has a reduced genome, making its proteins, including MG281. critical for survival and host interaction. The MG281 gene encodes a conserved adhesion-related protein implicated in bacterial attachment to host epithelial cells, a key step in colonization and pathogenesis.
Produced via recombinant DNA technology, the MG281 protein is typically expressed in *Escherichia coli* systems, enabling scalable and cost-effective purification. The recombinant form retains functional epitopes, allowing researchers to study its biological role without handling the fastidious native bacterium. Structurally, MG281 contains domains associated with membrane localization and potential immunogenic motifs, making it a target for diagnostic and therapeutic development.
Research on MG281 focuses on its contribution to *M. genitalium* virulence, including immune evasion mechanisms and interactions with host receptors. Its recombinant version is widely used in serological assays to detect antibodies in infected individuals, aiding diagnosis. Additionally, it serves as a candidate antigen in vaccine studies and a tool for elucidating adhesion pathways in microbial pathogenesis.
Beyond infectious disease, MG281 has applications in structural biology due to its compact, stable fold, offering insights into protein-minimalist adaptation. Ongoing studies explore its potential as a drug target to disrupt bacterial adhesion, addressing antibiotic resistance challenges. Overall, MG281 exemplifies the integration of recombinant protein technology in understanding pathogenicity and advancing biomedical solutions.
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