| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Trpc1 |
| Uniprot No | P48995 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-793aa |
| 氨基酸序列 | MMAALYPSTDLSGASSSSLPSSPSSSSPNEVMALKDVREVKEENTLNEKLFLLACDKGDY YMVKKILEENSSGDLNINCVDVLGRNAVTITIENENLDILQLLLDYGCQSADALLVAIDS EVVGAVDILLNHRPKRSSRPTIVKLMERIQNPEYSTTMDVAPVILAAHRNNYEILTMLLK QDVSLPKPHAVGCECTLCSAKNKKDSLRHSRFRLDIYRCLASPALIMLTEEDPILRAFEL SADLKELSLVEVEFRNDYEELARQCKMFAKDLLAQARNSRELEVILNHTSSDEPLDKRGL LEERMNLSRLKLAIKYNQKEFVSQSNCQQFLNTVWFGQMSGYRRKPTCKKIMTVLTVGIF WPVLSLCYLIAPKSQFGRIIHTPFMKFIIHGASYFTFLLLLNLYSLVYNEDKKNTMGPAL ERIDYLLILWIIGMIWSDIKRLWYEGLEDFLEESRNQLSFVMNSLYLATFALKVVAHNKF HDFADRKDWDAFHPTLVAEGLFAFANVLSYLRLFFMYTTSSILGPLQISMGQMLQDFGKF LGMFLLVLFSFTIGLTQLYDKGYTSKEQKDCVGIFCEQQSNDTFHSFIGTCFALFWYIFS LAHVAIFVTRFSYGEELQSFVGAVIVGTYNVVVVIVLTKLLVAMLHKSFQLIANHEDKEW KFARAKLWLSYFDDKCTLPPPFNIIPSPKTICYMISSLSKWICSHTSKGKVKRQNSLKEW RNLKQKRDENYQKVMCCLVHRYLTSMRQKMQSTDQATVENLNELRQDLSKFRNEIRDLLG FRTSKYAMFYPRN |
| 预测分子量 | 91,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRPC1重组蛋白的3篇示例参考文献(注：部分为模拟文献，建议通过学术数据库核实具体信息)：
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1. **文献名称**: *"Functional reconstitution of human TRPC1 into lipid bilayers reveals its role in store-operated calcium entry"*
**作者**: Yuan JP, Zeng W, et al.
**摘要**: 研究通过重组表达人源TRPC1蛋白，并将其重构至脂质双层中，证实TRPC1是储存操纵性钙通道(SOC)的核心组分，其活性受内质网钙耗竭调控，为钙信号传导机制提供直接证据。
2. **文献名称**: *"Expression and structural analysis of the TRPC1 ion channel using a baculovirus system"*
**作者**: Liao Y, Soom M, et al.
**摘要**: 利用杆状病毒系统在昆虫细胞中高效表达TRPC1重组蛋白，并通过冷冻电镜解析其跨膜结构域，揭示其四聚体组装模式及潜在的离子传导路径。
3. **文献名称**: *"Recombinant TRPC1 enhances cellular proliferation in breast cancer models via Ca²⁺-dependent signaling"*
**作者**: Singh BB, Liu X, et al.
**摘要**: 研究发现，过表达重组TRPC1蛋白可激活钙依赖性激酶(如CaMKII)，促进乳腺癌细胞增殖，提示TRPC1可能作为癌症治疗的潜在靶点。
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**备注**：上述文献为示例性质，实际引用时建议通过PubMed或Google Scholar以关键词“TRPC1 recombinant protein”检索最新研究。真实文献可能涉及重组TRPC1在疾病模型、结构解析或信号通路中的具体作用。
**Background of TRPC1 Recombinant Protein**
TRPC1 (Transient Receptor Potential Canonical 1) is a member of the TRP ion channel family, which plays a critical role in mediating calcium (Ca²⁺) and sodium (Na⁺) influx across cellular membranes. As a non-selective cation channel, TRPC1 is ubiquitously expressed in various tissues, including the brain, heart, and smooth muscle, and is involved in diverse physiological processes such as cell proliferation, apoptosis, and sensory transduction. Its activity is often regulated by G protein-coupled receptors (GPCRs) or receptor tyrosine kinases (RTKs), linking extracellular signals to intracellular Ca²⁺ dynamics.
The recombinant TRPC1 protein is engineered through molecular cloning and heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its structure-function relationships, regulatory mechanisms, and interactions with other proteins. Recombinant TRPC1 retains key structural features, including six transmembrane domains, a pore-forming region, and cytosolic N- and C-terminal domains involved in channel assembly and modulation.
Research utilizing TRPC1 recombinant protein has advanced understanding of its role in pathologies such as neurodegenerative diseases, cardiovascular disorders, and cancer. For instance, dysregulated TRPC1 expression or function has been implicated in hypertension, cardiac hypertrophy, and tumor metastasis. The recombinant protein serves as a valuable tool for drug discovery, enabling high-throughput screening of channel modulators.
Production of TRPC1 recombinant protein typically involves affinity chromatography and tag-based purification (e.g., His-tag), followed by validation via SDS-PAGE, Western blotting, and functional assays (e.g., electrophysiology or Ca²⁺ imaging). Challenges include maintaining proper folding and post-translational modifications critical for channel activity.
Overall, TRPC1 recombinant protein is pivotal for elucidating its biological significance and therapeutic potential in Ca²⁺-associated diseases.
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