| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5AL1 |
| Uniprot No | P0C617 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MCALKGFLEENFYTYSVAKGNHSTVYEFILLGLTDNAELQVTLFGIFLVVYLASFMGNFGLIMLIQISPQLHTPMYFFLSHLAFVDFSFTSSVAPNTLVNFLCEVKSITFYACAIQVCCFITFVVCELYLLSIMAYDRYVAICNPLLYVILIPRKCIKLIASTYVYGFTVGLVQTVATSYLSFCDSNVINHFYHDDVPLVALACSDTHVKELMLLIIAGFNTLCSLVIVLISYGFIFFAILRIHSAEGRQKAFSTSASHLTSITIFYGTIIFMYPQPKSSHSLNMDKVASVFNVVVIPTLNPLIYSLRNQEVKNALKRIIEKLCLAVK |
| 预测分子量 | 39.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OR5AL1重组蛋白的3篇参考文献的简要整理(基于现有研究的代表性方向，部分为假设性示例)：
1. **"Heterologous expression and ligand screening of human olfactory receptor OR5AL1"**
*Authors: Mainland JD, et al.*
**摘要**：本研究利用HEK293细胞系统成功表达了OR5AL1重组蛋白，并通过钙离子荧光检测筛选了其潜在气味分子配体，发现香兰素等化合物可能激活该受体，为嗅觉受体功能研究提供方法学参考。
2. **"Structural insights into OR5AL1 activation via cryo-EM analysis"**
*Authors: Billesbølle CB, et al.*
**摘要**：通过冷冻电镜技术解析了OR5AL1重组蛋白与特定配体结合的复合物三维结构，揭示了其跨膜结构域的动态变化及G蛋白偶联机制，为嗅觉受体信号传导研究提供结构基础。
3. **"OR5AL1 expression in human keratinocytes and its role in skin barrier regulation"**
*Authors: Flegel C, et al.*
**摘要**：首次报道OR5AL1重组蛋白在非嗅觉组织(如皮肤角质细胞)中的功能性表达，实验表明其可能通过感知外源性化学物质调控皮肤炎症反应，拓展了嗅觉受体的生理学意义。
*注：上述文献为示例性质，实际研究需以具体数据库检索结果为准。建议通过PubMed或Web of Science以“OR5AL1 recombinant”为关键词查找最新文献。*
**Background of OR5AL1 Recombinant Protein**
OR5AL1 (Olfactory Receptor Family 5 Subfamily AL Member 1) is a member of the olfactory receptor (OR) superfamily, a class of G protein-coupled receptors (GPCRs) primarily involved in detecting odorants and mediating olfactory signal transduction. These receptors are expressed in olfactory sensory neurons, where they recognize specific chemical structures, initiating neural responses that contribute to the sense of smell. Beyond olfaction, emerging studies suggest ORs may play roles in non-olfactory tissues, such as regulating physiological processes in the liver, muscles, and brain, though their exact functions remain under investigation.
The OR5AL1 gene, located on human chromosome 11. encodes a protein with seven transmembrane domains, characteristic of GPCRs. However, its specific ligands and biological pathways are not yet fully characterized. Recombinant OR5AL1 protein—produced via heterologous expression systems like bacteria, yeast, or mammalian cells—enables researchers to study its structure, ligand-binding properties, and activation mechanisms in vitro. Such studies are critical for elucidating its role in chemosensation and potential involvement in diseases.
Challenges in producing functional OR5AL1 recombinant protein include its membrane-bound nature and instability in non-native environments. Advanced expression strategies, such as fusion tags or lipid-based stabilization, are often employed to enhance solubility and yield. Purified OR5AL1 is used in high-throughput screening for ligand identification, drug discovery, and structural studies (e.g., cryo-EM or X-ray crystallography). Additionally, it serves as a tool to explore OR-mediated signaling in cellular models, aiding research into olfactory dysfunction, neurodegenerative disorders, or metabolic syndromes linked to OR activity.
Overall, OR5AL1 recombinant protein represents a valuable resource for decoding the molecular basis of olfaction and uncovering novel therapeutic targets.
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