| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DLK; FA1; ZOG; pG2; PREF1; Pref-1 |
| Entrez GeneID | 8788 |
| clone | 3A10 |
| WB Predicted band size | 41kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DLK1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DLK1抗体的3篇参考文献示例(注：文献信息为虚拟示例，实际引用需查询真实数据库)：
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1. **文献名称**：*DLK1 as a Serum Biomarker for Adrenocortical Carcinoma*
**作者**：Smith A, et al.
**摘要内容**：本研究通过免疫组化及Western blot分析，利用DLK1特异性抗体验证了DLK1在肾上腺皮质癌组织中的高表达，提示其可作为潜在的诊断标志物。抗体特异性通过siRNA敲除实验确认。
2. **文献名称**：*Role of DLK1 in Adipocyte Differentiation*
**作者**：Lee B, et al.
**摘要内容**：通过抗DLK1抗体阻断实验，发现DLK1在抑制前脂肪细胞分化中起关键作用。研究采用流式细胞术和免疫荧光技术，证实DLK1抗体可逆转分化抑制表型。
3. **文献名称**：*DLK1 Expression in Glioblastoma Stem Cells*
**作者**：Chen C, et al.
**摘要内容**：利用DLK1单克隆抗体对胶质母细胞瘤样本进行染色，发现DLK1在肿瘤干细胞亚群中特异性高表达，提示其参与肿瘤干性维持及耐药机制。
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**备注**：以上文献信息为示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献(可尝试关键词：DLK1 antibody, DLK1 immunohistochemistry, DLK1 biomarker)。若需具体文献协助，请提供更多研究背景或应用方向。
Delta-like 1 homolog (DLK1), also known as preadipocyte factor 1 (Pref-1), is a transmembrane protein belonging to the epidermal growth factor (EGF)-like repeat protein family. It plays critical roles in cell differentiation, tissue development, and metabolic regulation. DLK1 is highly expressed during embryogenesis, particularly in developing tissues such as adipose, muscle, and endocrine organs, but its expression diminishes in most adult tissues except certain stem cell niches. Aberrant DLK1 expression is linked to metabolic disorders (e.g., obesity, diabetes), cancers (e.g., neuroendocrine tumors, hepatoblastoma), and developmental abnormalities.
DLK1 antibodies are essential tools for studying its biological functions and pathological roles. They enable the detection of DLK1 in tissues or cells via techniques like immunohistochemistry, Western blotting, and flow cytometry. These antibodies often target extracellular EGF-like repeats or intracellular domains, distinguishing membrane-bound DLK1 from its soluble form. In research, DLK1 antibodies help elucidate mechanisms in adipogenesis, tumorigenesis, and stem cell maintenance. Clinically, they hold diagnostic potential as DLK1 is a biomarker for某些 cancers and metabolic diseases. Therapeutic applications are also being explored, including antibody-based targeting to inhibit DLK1 signaling in cancers or modulate adipocyte differentiation. Commercial DLK1 antibodies vary in specificity and host species, requiring validation for experimental reproducibility.
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