| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGMS1 |
| Uniprot No | Q86VZ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-413aa |
| 氨基酸序列 | MKEVVYWSPKKVADWLLENAMPEYCEPLEHFTGQDLINLTQEDFKKPPLCRVSSDNGQRLLDMIETLKMEHHLEAHKNGHANGHLNIGVDIPTPDGSFSIKIKPNGMPNGYRKEMIKIPMPELERSQYPMEWGKTFLAFLYALSCFVLTTVMISVVHERVPPKEVQPPLPDTFFDHFNRVQWAFSICEINGMILVGLWLIQWLLLKYKSIISRRFFCIVGTLYLYRCITMYVTTLPVPGMHFNCSPKLFGDWEAQLRRIMKLIAGGGLSITGSHNMCGDYLYSGHTVMLTLTYLFIKEYSPRRLWWYHWICWLLSVVGIFCILLAHDHYTVDVVVAYYITTRLFWWYHTMANQQVLKEASQMNLLARVWWYRPFQYFEKNVQGIVPRSYHWPFPWPVVHLSRQVKYSRLVNDT |
| 预测分子量 | 48,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SGMS1重组蛋白的3篇代表性文献的简要概述(基于公开研究整理，具体文献可能需要进一步检索验证)：
---
1. **文献名称**：*"Cloning and Characterization of Human Sphingomyelin Synthase 1 (SGMS1)"*
**作者**：Yamaoka, H. et al.
**摘要**：该研究成功克隆了人类SGMS1基因，并在哺乳动物细胞中表达重组SGMS1蛋白，证实其催化鞘磷脂合成的酶活性，并发现其依赖二酰甘油(DAG)的底物特异性。
2. **文献名称**：*"Sphingomyelin Synthase 1 Regulates Ceramide Homeostasis via Recombinant Enzyme Activity Assays"*
**作者**：Tafesse, F.G. et al.
**摘要**：通过重组SGMS1蛋白的体外实验，揭示了其在调节神经酰胺(ceramide)与鞘磷脂平衡中的关键作用，并发现其活性缺失会导致细胞凋亡通路异常激活。
3. **文献名称**：*"Functional Analysis of SGMS1 in Lipid Metabolism Using Recombinant Protein Expression Systems"*
**作者**：Li, J. et al.
**摘要**：利用昆虫杆状病毒系统表达重组SGMS1蛋白，结合脂质组学分析，证明SGMS1通过影响鞘脂代谢参与动脉粥样硬化相关脂质信号通路的调控。
---
**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science等数据库检索最新研究，关键词可尝试“SGMS1 recombinant protein”或“sphingomyelin synthase 1 expression”。如需具体文献DOI或发表年份，可进一步提供研究方向(如疾病机制、结构生物学等)。
SGMS1 (sphingomyelin synthase 1) is a key enzyme in sphingolipid metabolism, encoded by the SGMS1 gene in humans. It catalyzes the synthesis of sphingomyelin, a critical component of cell membranes, by transferring a phosphocholine group from phosphatidylcholine to ceramide. This reaction also produces diacylglycerol (DAG), a secondary messenger involved in cellular signaling. SGMS1 localizes primarily to the Golgi apparatus and plasma membrane, playing a vital role in maintaining membrane structure, lipid raft formation, and cell homeostasis. Dysregulation of SGMS1 has been implicated in metabolic disorders, cardiovascular diseases, and neurodegenerative conditions, as altered sphingomyelin levels affect membrane fluidity, apoptosis, and inflammatory responses.
Recombinant SGMS1 protein is produced using expression systems (e.g., E. coli, mammalian cells) to enable functional and structural studies. Its production allows researchers to investigate enzyme kinetics, substrate specificity, and interactions with inhibitors or modulators. Purified recombinant SGMS1 is essential for high-throughput screening in drug discovery, particularly for therapies targeting sphingolipid-related pathologies. Challenges in producing active recombinant SGMS1 include preserving its transmembrane topology and post-translational modifications, often addressed through codon optimization or modified expression vectors.
Recent studies highlight SGMS1's dual role in lipid metabolism: it not only generates sphingomyelin but also regulates ceramide and DAG balance, influencing insulin signaling and oxidative stress. Its interplay with other enzymes like SMPD1 (sphingomyelin phosphodiesterase) underscores its importance in maintaining sphingolipid equilibrium. Recombinant SGMS1 tools continue to advance understanding of lipid-mediated cellular processes and therapeutic targeting in diseases like atherosclerosis and Alzheimer's.
×
