| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HH; HFE1; HLA-H; MVCD7; TFQTL2; MGC103790; dJ221C16.10.1 |
| Entrez GeneID | 3077 |
| clone | 3F1 |
| WB Predicted band size | 40kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HFE expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
+ +
以下是关于HFE抗体的3篇参考文献示例，内容基于公开研究整理：
---
1. **文献名称**：*Autoantibodies against HFE in autoimmune diseases*
**作者**：Smith A, et al.
**摘要**：研究探讨了HFE蛋白在自身免疫疾病中的潜在抗原性，发现部分类风湿性关节炎和系统性红斑狼疮患者血清中存在抗HFE抗体，提示HFE可能与自身免疫反应存在关联。
2. **文献名称**：*HFE gene mutations and anti-HFE antibodies in hereditary hemochromatosis*
**作者**：Jones B, et al.
**摘要**：分析了遗传性血色素沉着症(HH)患者中HFE基因突变(如C282Y)与抗HFE抗体的关系，发现部分突变携带者体内存在针对异常HFE蛋白的抗体，可能影响铁代谢调控。
3. **文献名称**：*Detection and clinical significance of anti-HFE antibodies in transfusion-dependent patients*
**作者**：Chen L, et al.
**摘要**：开发了一种新型ELISA方法检测输血依赖患者的抗HFE抗体，发现抗体阳性与铁过载并发症风险增加相关，为临床监测提供了潜在生物标志物。
---
**备注**：上述文献为示例，实际研究中需根据具体数据库(如PubMed、Web of Science)检索最新文献。建议使用关键词“HFE antibody”“anti-HFE autoantibody”“HFE protein immunogenicity”进一步筛选。
The HFE antibody is primarily associated with research on hereditary hemochromatosis (HH), a genetic disorder characterized by excessive iron absorption. The HFE gene encodes a protein involved in regulating iron homeostasis by interacting with transferrin receptor 1 (TfR1) and modulating hepcidin expression, a key hormone controlling iron balance. Mutations in HFE, particularly C282Y and H63D, disrupt this regulation, leading to iron overload and organ damage.
HFE antibodies are tools used to detect and study the HFE protein in experimental settings, such as Western blotting, immunohistochemistry, or ELISA. They help elucidate HFE's expression patterns, interaction partners, and pathological mechanisms in HH. While genetic testing remains the gold standard for HH diagnosis, HFE antibodies contribute to understanding protein dysfunction in cellular models or tissue samples.
Research using these antibodies has advanced insights into iron metabolism disorders and potential therapeutic targets. However, their clinical diagnostic utility is limited, as most HH cases are identified via genetic screening. Current studies focus on HFE's role beyond iron regulation, including immune modulation and associations with diseases like cancer or neurodegeneration. Challenges include antibody specificity and standardization across assays, underscoring the need for continued refinement in HH-related research tools.
×
