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| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | Ilp7 |
| Uniprot No | Q9W4Q9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-159aa |
| 氨基酸序列 | LQHTEEGLEMLFRERSQSDWENVWHQETHSRCRDKLVRQLYWACEKDIYRLTRRNKKRTGNDEAWIKKTTTEPDGSTWLHVNYANMFLRSRRSDGNTPSISNECCTKAGCTWEEYAEYCPSNKRRNHY |
| 预测分子量 | 19.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ilp7重组蛋白的3篇代表性文献的模拟示例(具体文献需根据实际研究补充核实)：
1. **文献名称**: *Drosophila Insulin-like Peptide 7 (Ilp7) Regulates Oocyte Maturation via a Neuronal Signaling Pathway*
**作者**: S. Slaidina, et al.
**摘要**: 本研究利用重组Ilp7蛋白揭示了其在果蝇卵母细胞成熟中的关键作用，证实Ilp7通过激活中枢神经系统的胰岛素信号通路调控生殖周期。
2. **文献名称**: *Recombinant Expression and Functional Characterization of Ilp7 in Tribolium castaneum*
**作者**: Y. Okamoto, et al.
**摘要**: 通过大肠杆菌系统成功表达并纯化Ilp7重组蛋白，发现其可结合昆虫肠道受体，调控蜕皮激素合成，为害虫防治提供潜在靶点。
3. **文献名称**: *Structural Analysis of Recombinant Ilp7 Reveals Conserved Insulin-like Motifs*
**作者**: M. Tanaka, et al.
**摘要**: 采用X射线晶体学解析Ilp7重组蛋白的三维结构，发现其保留胰岛素家族核心结构域，但C端存在独特功能区域，可能参与特异性受体结合。
4. **文献名称**: *Ilp7 Recombinant Protein Enhances Drug Delivery Efficiency in a Cancer Model*
**作者**: J. Lee, et al.
**摘要**: 通过融合Ilp7重组蛋白与纳米颗粒，显著提高靶向肿瘤细胞的药物递送效率，证明其在生物医学应用中的潜力。
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**注意**：以上文献为示例性质，实际研究中需以具体数据库(如PubMed、Web of Science)检索结果为准。若需真实文献，建议补充关键词(如物种、研究领域)或提供初步检索方向。
**Background of ILP7 Recombinant Protein**
Insulin-like peptide 7 (ILP7) is a member of the insulin/insulin-like growth factor (IGF) signaling pathway, a highly conserved system critical for regulating growth, metabolism, reproduction, and longevity across species. In *Drosophila melanogaster*, ILP7 is specifically expressed in neurosecretory cells of the brain and plays a role in coordinating developmental and metabolic processes. Unlike other insulin-like peptides in flies, ILP7 is implicated in stress response and reproductive regulation, though its precise mechanisms remain under investigation.
Recombinant ILP7 protein is produced using biotechnological methods, such as bacterial or mammalian expression systems, to enable functional studies. The recombinant form allows researchers to explore ILP7's binding affinities, receptor interactions, and downstream signaling pathways *in vitro* or in model organisms. Its production typically involves cloning the *ilp7* gene into expression vectors, followed by protein purification via affinity chromatography (e.g., His-tag systems).
Interest in ILP7 stems from its potential to elucidate conserved insulin signaling mechanisms relevant to human diseases, including diabetes, metabolic disorders, and age-related conditions. Studies using recombinant ILP7 have also shed light on its role in adapting to nutrient deprivation or oxidative stress, providing insights into evolutionary strategies for survival. Additionally, ILP7's tissue-specific effects make it a valuable tool for dissecting the complexity of insulin-related networks.
Overall, recombinant ILP7 serves as a key reagent for advancing understanding of endocrine regulation and developing targeted therapies for metabolic dysregulation.
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