| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | E398K |
| Uniprot No | P06731 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-685aa |
| 氨基酸序列 | KLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNKLSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSA |
| 预测分子量 | 73.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于E398K重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
---
1. **文献名称**：*Structural and Functional Impact of E398K Mutation in Recombinant ABCG2 Protein*
**作者**：Smith J, et al.
**摘要**：本研究通过X射线晶体学分析了E398K突变对ABCG2转运蛋白结构的影响，发现该突变导致跨膜结构域构象变化，降低其底物外排活性，可能解释与化疗耐药性相关的临床表型。
---
2. **文献名称**：*E398K Variant in Recombinant CFTR: Implications for Chloride Channel Dysfunction*
**作者**：Lee H, et al.
**摘要**：利用电生理学方法验证E398K突变对重组CFTR蛋白氯离子通道功能的影响，结果显示突变体通道开放概率降低50%，为囊性纤维化患者的个性化治疗提供分子机制依据。
---
3. **文献名称**：*Kinetic Analysis of E398K-Mutant Recombinant Tyrosine Kinase in Cancer Cell Signaling*
**作者**：Garcia R, et al.
**摘要**：通过酶动力学实验证明，E398K突变显著增强重组酪氨酸激酶的自磷酸化能力，并激活下游MAPK通路，提示该突变可能作为肿瘤靶向治疗的潜在生物标志物。
---
4. **文献名称**：*E398K Recombinant Amyloid-β Protein Aggregation in Alzheimer's Disease Models*
**作者**：Wang Y, et al.
**摘要**：研究利用体外重组技术表达E398K突变的β-淀粉样蛋白，发现其纤维聚集速率较野生型提高2倍，且毒性增强，为早发性阿尔茨海默病的病理机制提供新见解。
---
**注**：以上文献为模拟生成，实际研究中需通过学术数据库(如PubMed、Web of Science)检索真实文献。若需具体领域文献，可进一步补充蛋白名称或疾病背景优化检索。
The E398K recombinant protein is a genetically engineered variant of a parent protein where the glutamic acid (E) at position 398 is replaced by lysine (K). This substitution is typically introduced to study the functional or structural impact of this specific residue on the protein's activity, stability, or interactions. The "E398K" mutation may arise in natural genetic variants linked to diseases or engineered in laboratory settings to explore molecular mechanisms. For instance, such mutations are often investigated in enzymes, receptors, or signaling proteins where residue 398 lies within a critical domain, such as an active site, allosteric region, or binding interface.
Recombinant E398K proteins are commonly produced using expression systems like *E. coli*, yeast, or mammalian cells, followed by purification via affinity tags (e.g., His-tag). Researchers analyze the mutant protein using biochemical assays (e.g., enzymatic activity, ligand binding), biophysical techniques (e.g., circular dichroism, thermal shift assays), or structural methods (X-ray crystallography, cryo-EM) to compare its properties with the wild-type protein. Such studies aim to unravel how the E-to-K substitution alters charge, hydrogen bonding, or conformational dynamics, potentially affecting cellular pathways or disease phenotypes. For example, in disease-associated proteins (e.g., kinases, tumor suppressors), the E398K mutation might impair function, leading to pathological outcomes, or enhance stability for therapeutic applications. This variant also serves as a tool for drug discovery, helping identify inhibitors targeting mutation-specific conformations. Overall, E398K recombinant proteins bridge genetic observations to mechanistic insights, advancing both basic science and translational research.
×
