| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCBP1 |
| Uniprot No | Q09161 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-790aa |
| 氨基酸序列 | MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGVLEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA |
| 预测分子量 | 95.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于NCBP1(核帽结合蛋白1)重组蛋白的参考文献及简要摘要：
1. **"Structural characterization of the CBP80-CBP20 complex bound to m7GpppG"**
- **作者**: Mazza et al. (2001)
- **摘要**: 研究利用重组表达的CBP80(NCBP1)和CBP20蛋白解析其与mRNA 5'端帽结构(m7GpppG)的复合物晶体结构，揭示了两者协同识别RNA帽的分子机制。
2. **"NCBP1 interacts with the exon junction complex and participates in nonsense-mediated mRNA decay"**
- **作者**: Sato & Maquat (2003)
- **摘要**: 通过重组蛋白实验证明NCBP1与外显子连接复合物(EJC)相互作用，并参与无义介导的mRNA降解(NMD)过程，表明其在RNA质量控制中的功能。
3. **"The RNA-binding activity of NCBP1 is required for its role in nuclear mRNA export"**
- **作者**: Kataoka et al. (2000)
- **摘要**: 利用重组NCBP1蛋白进行体外RNA结合实验，发现其RNA结合活性对介导mRNA从细胞核到细胞质的转运至关重要。
4. **"Cloning and functional analysis of recombinant NCBP1 in mammalian cells"**
- **作者**: Gudkov et al. (1994)
- **摘要**: 首次报道人源NCBP1的克隆及重组表达，证实其在哺乳动物细胞中通过与RNA帽结合促进mRNA剪接和翻译起始的功能。
注：以上文献信息为示例性概括，具体内容需根据实际发表论文调整。建议通过PubMed或Google Scholar以“NCBP1 recombinant protein”为关键词检索最新研究。
**Background of NCBP1 Recombinant Protein**
NCBP1 (Nuclear Cap-Binding Protein Subunit 1) is a critical component of the cap-binding complex (CBC), a highly conserved eukaryotic protein complex that recognizes the 5ʹ-7-methylguanosine (m⁷G) cap structure of nascent RNA polymerase II transcripts. This recognition is essential for multiple RNA-processing steps, including pre-mRNA splicing, nuclear export, and translation initiation. NCBP1 partners with NCBP2 (or its alternative CBP80/20 isoforms) to form the heterodimeric CBC, which stabilizes the cap structure and recruits downstream effector proteins to regulate mRNA metabolism.
The gene encoding NCBP1 is evolutionarily conserved and ubiquitously expressed, underscoring its fundamental role in cellular RNA biology. Dysregulation of NCBP1 has been linked to aberrant RNA processing, which may contribute to diseases such as cancer, viral infections (where pathogens hijack host mRNA machinery), and neurodevelopmental disorders. For instance, NCBP1 mutations or altered expression can disrupt mRNA export or translation, impacting cell proliferation and differentiation.
Recombinant NCBP1 protein is produced *in vitro* using expression systems like *E. coli* or mammalian cells, enabling studies on its structure, interactions, and functional mechanisms. Purified NCBP1 retains cap-binding activity and is widely used to investigate CBC-dependent RNA processing pathways *in vitro* or in cellular assays. It also serves as a tool for screening therapeutic compounds targeting cap-dependent processes, such as viral replication or oncogenic translation. Structural studies using recombinant NCBP1 have revealed insights into its domain organization, including its RNP (RNA recognition motif) and its role in coordinating with NCBP2 for cap recognition.
Overall, NCBP1 recombinant protein is a vital reagent for dissecting the molecular basis of mRNA maturation and its implications in health and disease.
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