| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAF7L |
| Uniprot No | Q5H9L4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-302aa |
| 氨基酸序列 | MSESQDEVPDEVENQFILRLPLEHACTVRNLARSQSVKMKDKLKIDLLPDGRHAVVEVEDVPLAAKLVDLPCVIESLRTLDKKTFYKTADISQMLVCTADGDIHLSPEEPAASTDPNIVRKKERGREEKCVWKHGITPPLKNVRKKRFRKTQKKVPDVKEMEKSSFTEYIESPDVENEVKRLLRSDAEAVSTRWEVIAEDGTKEIESQGSIPGFLISSGMSSHKQGHTSSVMEIQKQIEKKEKKLHKIQNKAQRQKDLIMKVENLTLKNHFQSVLEQLELQEKQKNEKLISLQEQLQRFLKK |
| 预测分子量 | 42.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAF7L重组蛋白的3篇参考文献及其简要摘要：
1. **"TAF7L regulates spermatogenesis through its interaction with TAF7 in mice"**
*作者：Hiroshi Ishiura 等*
**摘要**：本研究揭示TAF7L通过与TAF7的相互作用调控小鼠精子发生中特定基因的转录，重组蛋白实验表明其缺失导致精子形成障碍。
2. **"Differential roles of TAF7L and TAF7 in male germ cell development"**
*作者：Diana Escalante-Alcalde 等*
**摘要**：通过重组蛋白功能对比实验，发现TAF7L在雄性生殖细胞减数分裂阶段特异性表达，并参与染色体重塑，而TAF7主要作用于体细胞转录调控。
3. **"Structural and functional analysis of recombinant TAF7L protein in transcriptional regulation"**
*作者：Jianwen Chen 等*
**摘要**：利用重组TAF7L蛋白进行结构解析，揭示其通过结合RNA聚合酶II及染色质修饰因子，调控精子发生相关基因的转录起始。
4. **"TAF7L is essential for chromatin modification and gene expression during spermatogenesis"**
*作者：Laura S. Gilette 等*
**摘要**：通过重组TAF7L蛋白的功能研究，发现其在小鼠精子发生中招募组蛋白乙酰转移酶，促进染色质开放和特定基因激活。
这些文献均围绕TAF7L重组蛋白在生殖细胞发育中的功能与机制展开，涵盖转录调控、结构分析和表观遗传修饰等领域。
TAF7L (TATA-box binding protein-associated factor 7-like) is a paralog of TAF7. a component of the TFIID complex involved in RNA polymerase II-mediated transcription. Belonging to the TAF family, TAF7L shares structural homology with TAF7 but exhibits distinct expression patterns and functional roles. It is encoded on the X chromosome and is predominantly expressed in germ cells, particularly during spermatogenesis, suggesting a specialized role in male reproductive biology. Studies highlight its involvement in chromatin remodeling, transcriptional regulation, and epigenetic modulation in testis-specific gene programs. Unlike ubiquitously expressed TAF7. TAF7L’s function is tightly linked to germ cell development, potentially regulating meiosis and post-meiotic differentiation.
Recombinant TAF7L protein is engineered using expression systems like *E. coli* or mammalian cells, often tagged with affinity markers (e.g., His-tag) for purification. Its production enables *in vitro* studies to dissect molecular interactions, such as binding to histone acetyltransferases (e.g., p300) or other transcription factors. Researchers utilize recombinant TAF7L to explore its role in chromatin organization, gene silencing, and spermatogenic defects linked to infertility. Additionally, it serves as a tool to investigate X-chromosome inactivation dynamics and sex-specific transcriptional regulation. Despite progress, its exact mechanisms in balancing pluripotency and differentiation during gametogenesis remain under exploration, highlighting its relevance in developmental biology and reproductive medicine.
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