| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ASK1; MEKK5; MAPKKK5 |
| Entrez GeneID | 4217 |
| clone | 2E4 |
| WB Predicted band size | 55kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP3K5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于 **MAP3K5抗体** 的参考文献示例(内容为模拟虚构，仅供参考)：
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1. **文献名称**: *MAP3K5 regulates cardiac hypertrophy through JNK signaling pathway*
**作者**: Kim, S. T. et al.
**摘要**: 本研究通过Western blot和免疫组化实验，发现MAP3K5抗体特异性检测到心肌细胞中MAP3K5的表达水平升高与心脏肥厚相关，提示其在JNK通路中的调控作用。
2. **文献名称**: *MAP3K5 as a prognostic biomarker in hepatocellular carcinoma*
**作者**: Zhang, J. et al.
**摘要**: 利用MAP3K5抗体对肝癌组织进行免疫染色，发现MAP3K5高表达与患者不良预后显著相关，可能通过激活下游ERK通路促进肿瘤侵袭。
3. **文献名称**: *MAP3K5 mediates tau phosphorylation in Alzheimer's disease models*
**作者**: Smith, A. R. et al.
**摘要**: 通过siRNA沉默和MAP3K5抗体验证，证明MAP3K5参与Aβ诱导的tau蛋白异常磷酸化，为阿尔茨海默病的神经炎症机制提供新证据。
4. **文献名称**: *Validation of commercial MAP3K5 antibodies for specificity in human tissues*
**作者**: Tanaka, M. et al.
**摘要**: 系统评估了市售MAP3K5抗体在流式细胞术和免疫沉淀中的特异性，发现克隆号AB1234在多种组织样本中表现出高灵敏度和低交叉反应性。
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注：以上文献为示例，实际引用需查询真实数据库(如PubMed)。若需真实文献，建议通过关键词“MAP3K5 antibody”或“MEKK5 antibody”在学术平台检索。
The MAP3K5 (Mitogen-Activated Protein Kinase Kinase Kinase 5), also known as ASK1 (Apoptosis Signal-Regulating Kinase 1), is a serine/threonine kinase involved in stress-activated signaling pathways. It plays a critical role in mediating cellular responses to oxidative stress, endoplasmic reticulum stress, and inflammatory cytokines by activating downstream MAPK cascades, including JNK and p38 pathways. MAP3K5 is implicated in apoptosis, differentiation, and inflammation, with its dysregulation linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
MAP3K5 antibodies are essential tools for studying its expression, activation, and interaction networks. These antibodies are typically generated using immunogens derived from specific regions of the human MAP3K5 protein, such as the kinase domain or N-/C-terminal regions. They enable detection of MAP3K5 in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Validation often includes verifying molecular weight (~170 kDa) and specificity via knockout controls or siRNA knockdown.
Research applications focus on understanding MAP3K5's role in stress-induced apoptosis, its regulation by thioredoxin (which inhibits its activity under redox balance), and its crosstalk with other pathways like NF-κB. Commercial MAP3K5 antibodies may vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and epitope specificity, requiring careful selection based on experimental needs.
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