Mouse Monoclonal OTX2 Antibody

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

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     Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.    

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     Immunofluorescence analysis of U251 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

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     Immunohistochemical analysis of paraffin-embedded human colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded human stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.    

The OTX2 antibody is a crucial tool in developmental biology and oncological research, targeting the Orthodenticle Homeobox 2 (OTX2) protein, a transcription factor encoded by the OTX2 gene. OTX2 belongs to the bicoid-like homeobox family and plays a pivotal role in embryonic development, particularly in the formation of the forebrain, eyes, and pituitary gland. It regulates gene expression by binding to specific DNA sequences, guiding cell differentiation and tissue patterning. Dysregulation of OTX2 is implicated in various diseases, including congenital malformations (e.g., microphthalmia, pituitary defects) and cancers such as medulloblastoma, retinoblastoma, and neuroendocrine tumors, where its overexpression often correlates with aggressive phenotypes.

OTX2 antibodies are widely used in research to detect protein localization and expression levels via techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence (IF). These antibodies, often raised in rabbits or mice, can be monoclonal or polyclonal, with specificity validated through knockout controls or peptide blocking assays. Their applications extend to studying neural development, retinal disorders, and cancer mechanisms, aiding in the identification of OTX2-driven pathways or therapeutic targets. Recent studies also explore OTX2's role in maintaining cancer stem cells, highlighting its potential as a biomarker or drug target. However, challenges remain in standardizing antibody performance across experimental conditions, emphasizing the need for rigorous validation in diverse biological contexts.