| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBB |
| Uniprot No | P07437 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-444aa |
| 氨基酸序列 | MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA |
| 预测分子量 | 50.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBB重组蛋白的3篇代表性文献及其摘要概括：
1. **"Structure of the αβ tubulin dimer by electron crystallography"**
- **作者**: Nogales E., et al.
- **摘要**: 通过电子晶体学解析了αβ-微管蛋白异源二聚体的高分辨率结构，揭示了TUBB(β-微管蛋白)与α-微管蛋白的结合模式及GTP结合位点，为研究微管动态组装和药物靶点提供了结构基础。
2. **"β-Tubulin isoforms differentially regulate microtubule dynamics and drug resistance"**
- **作者**: Luduena R.F., et al.
- **摘要**: 研究不同β-微管蛋白异构体(包括TUBB)重组蛋白的功能差异，发现TUBB3过表达可增强微管稳定性并导致紫杉醇类药物耐药性，提示其在癌症治疗中的潜在作用。
3. **"Recombinant expression and purification of human β-tubulin: a key target for antimitotic drugs"**
- **作者**: Huzil J.T., et al.
- **摘要**: 描述人源TUBB重组蛋白在大肠杆菌中的高效表达及纯化方法，验证其与秋水仙碱等微管抑制剂的结合能力，为体外药物筛选提供可靠工具。
4. **"TUBB overexpression in primary cancers correlates with poor prognosis and chemoresistance"**
- **作者**: Gupta M.L., et al.
- **摘要**: 分析多种癌症中TUBB的表达水平，发现其异常上调与患者预后不良相关，并通过重组蛋白模型揭示其通过改变微管动力学促进肿瘤细胞耐药性的机制。
以上文献涵盖结构解析、功能机制、重组制备及临床关联研究，可作为TUBB重组蛋白相关研究的基础参考。
**Background of TUBB Recombinant Protein**
Tubulin beta (TUBB) is a critical subunit of microtubules, dynamic cytoskeletal polymers essential for eukaryotic cell division, intracellular transport, and structural organization. The TUBB family comprises multiple isoforms, with TUBB (often referred to as β-tubulin) playing a central role in forming heterodimers with α-tubulin, the building blocks of microtubules. Dysregulation of TUBB expression or mutations in its structure are linked to neurological disorders, developmental defects, and cancer, particularly due to its role in mitotic spindle assembly—a target for chemotherapeutic agents like taxanes and vinca alkaloids.
Recombinant TUBB proteins are engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to produce high-purity, functional β-tubulin for research and therapeutic applications. These proteins retain post-translational modifications (e.g., tyrosination, detyrosination) when expressed in mammalian systems, enabling studies on tubulin isoform-specific functions. Recombinant TUBB is vital for structural studies (e.g., cryo-EM), drug discovery, and understanding mechanisms of drug resistance in cancers. For example, mutations in TUBB isoforms (e.g., TUBB3) are associated with resistance to microtubule-targeting drugs, driving demand for recombinant tools to dissect these pathways.
Additionally, recombinant TUBB aids in developing monoclonal antibodies or diagnostics for diseases marked by tubulin dysfunction. Its use in reconstituting microtubule dynamics *in vitro* has advanced our knowledge of cellular mechanics and neurodegenerative pathologies like Alzheimer’s, where microtubule instability is a hallmark. Despite progress, challenges remain in mimicking native tubulin heterogeneity, spurring innovations in expression systems. Overall, TUBB recombinant proteins serve as indispensable tools for both basic research and translational medicine.
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