| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ERK3; PRKM6; p97MAPK; HsT17250; DKFZp686F03189; MAPK6 |
| Entrez GeneID | 5597 |
| clone | 4E8 |
| WB Predicted band size | 105kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ERK3 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于ERK3抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**：*ERK3/MAPK6 regulates cell motility through phosphorylation of the actin-binding protein cofilin*
**作者**：Smith J, et al.
**摘要**：本研究利用特异性ERK3抗体，通过Western blot和免疫荧光技术，揭示了ERK3通过磷酸化调控cofilin蛋白活性，从而影响癌细胞迁移的分子机制。
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2. **文献名称**：*Development and validation of a novel ERK3-specific monoclonal antibody for functional studies*
**作者**：Lee S, et al.
**摘要**：作者开发了一种高特异性ERK3单克隆抗体，并通过免疫沉淀和质谱分析验证其可靠性，为研究ERK3在细胞周期中的功能提供了关键工具。
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3. **文献名称**：*ERK3 interacts with the mTORC2 complex to modulate cellular stress responses*
**作者**：Garcia R, et al.
**摘要**：利用ERK3抗体进行共免疫沉淀实验，研究发现ERK3与mTORC2复合物相互作用，参与细胞在氧化应激条件下的存活调控。
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4. **文献名称**：*Differential expression of ERK3 in normal and malignant tissues revealed by immunohistochemistry*
**作者**：Wang Y, et al.
**摘要**：通过基于ERK3抗体的组织芯片免疫组化分析，发现ERK3在多种实体瘤中高表达，提示其作为潜在肿瘤标志物的可能性。
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**备注**：以上文献为示例性质，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar检索关键词“ERK3 antibody”或“MAPK6 antibody”获取最新研究。
**Background of ERK3 Antibody**
Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6. is a member of the mitogen-activated protein kinase (MAPK) family, which plays critical roles in transducing extracellular signals to intracellular responses. Unlike canonical MAPKs such as ERK1/2. ERK3 exhibits distinct structural and functional characteristics. It contains a unique C-terminal extension and lacks the conserved Thr-X-Tyr activation motif found in other MAPKs, instead harboring a Ser-Glu-Gly (SEG) activation loop. ERK3 is activated through phosphorylation, though its upstream regulators and downstream effectors remain less defined compared to other MAPKs.
ERK3 has been implicated in diverse cellular processes, including cell cycle regulation, differentiation, migration, and stress responses. Studies suggest its involvement in embryonic development, cancer progression, and immune regulation. For example, ERK3 interacts with the MK5/PRAK kinase, influencing cytoskeletal dynamics and cell motility. Its expression is often dysregulated in cancers, highlighting potential therapeutic relevance.
ERK3 antibodies are essential tools for studying its expression, localization, and activation. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. However, due to ERK3's homology with ERK4 (MAPK4), antibody specificity must be rigorously validated using knockout controls. Research on ERK3 is ongoing to clarify its signaling mechanisms and physiological roles, making reliable antibodies critical for advancing understanding in cellular and disease contexts.
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