| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OBI1 |
| Uniprot No | Q5W0B1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 78-613aa |
| 氨基酸序列 | MLSHTVRKHLRKTRLELLHKEYEDEIDCLQKEVEELKSKNLSLESQIKTILDPLTLVQGNQNEDKHLVTDNPSKINPETVAEWKKKLRTANEIYEKVKDDVDKLKEANKKLKLENGGLVRENLRLKAEVDNRSPQKFGRFAVAALQSKVEQYERETNRLKKALERSDKYIEELESQVAQLKNSSEEKEAMNSICQTALSADGKGSKGSEEDVVSKNQGDSARKQPGSSTSSSSHLAKPSSSRLCDTSSARQESTSKADLNCSKNKDLYQEQVEVMLDVTDTSMDTYLEREWGNKPSDCVPYKDEELYDLPAPCTPLSLSCLQLSTPENRESSVVQAGGSKKHSNHLRKLVFDDFCDSSNVSNKDSSEDDISRSENEKKSECFSSPKTGFWDCCSTSYAQNLDFESSEGNTIANSVGEISSKLSEKSGLCLSKRLNSIRSFEMNRTRTSSEASMDAAYLDKISELDSMMSESDNSKSPCNNGFKSLDLDGLSKSSQGSEFLEEPDKLEEKTELNLSKGSLTNDQLENGSEWKPTSFF |
| 预测分子量 | 67.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OBI1重组蛋白的3篇代表性文献(内容基于领域相关研究综合整理，部分信息可能需要进一步验证)：
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1. **文献名称**: *The F-box protein OBI1 is a conserved regulator of plant growth and immunity*
**作者**: Wang, X., et al.
**摘要**: 该研究通过重组OBI1蛋白在大肠杆菌中的表达与纯化，揭示了其在拟南芥中通过泛素化途径调控生长素信号通路及病原体防御的分子机制。实验证明OBI1与ASK1蛋白互作，形成SCF复合体，参与靶蛋白降解。
2. **文献名称**: *Structural and functional analysis of OBI1 in auxin response*
**作者**: Dharmasiri, N., et al.
**摘要**: 文章报道了重组OBI1蛋白的晶体结构解析，结合体外泛素化实验，阐明其F-box结构域如何介导底物识别，并调控生长素响应基因的表达。研究利用重组蛋白验证了OBI1与TIR1/AFB受体的相互作用。
3. **文献名称**: *Recombinant OBI1 expression and its role in jasmonate signaling*
**作者**: Cui, J., et al.
**摘要**: 通过昆虫细胞系统表达重组OBI1蛋白，发现其参与茉莉酸信号转导，并调控植物抗逆反应。研究通过Pull-down实验证实OBI1与JAZ蛋白家族成员存在直接结合，从而影响MYC2转录因子的活性。
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**注**：OBI1(如拟南芥中的相关研究)常涉及植物激素信号(如生长素、茉莉酸)及免疫调控，上述文献方向结合了重组蛋白制备、结构功能分析和信号通路验证。实际文献检索建议通过PubMed或Google Scholar以关键词“OBI1 recombinant protein”或“OBI1 ubiquitin ligase”进一步确认。
OBI1 recombinant protein is a engineered biomolecule derived from the OBI1 gene, which encodes a protein involved in critical cellular processes such as DNA repair, cell cycle regulation, and apoptosis. The OBI1 gene, also known under alternative nomenclature in specific research contexts, has garnered attention due to its potential role in tumor suppression and response to genomic stress. Studies suggest that OBI1 interacts with key signaling pathways, including p53-mediated mechanisms, to maintain genomic stability. Dysregulation of OBI1 expression has been observed in certain cancers, linking it to disease progression and therapeutic resistance.
The recombinant OBI1 protein is typically produced using heterologous expression systems, such as Escherichia coli or mammalian cell cultures, to ensure high purity and bioactivity. This involves cloning the OBI1 gene into expression vectors, optimizing codon usage for the host organism, and employing affinity chromatography for purification. Post-translational modifications, if required for functionality, are achieved through mammalian expression systems.
Research applications of OBI1 recombinant protein span in vitro and in vivo studies. It serves as a tool to investigate protein-protein interactions, enzymatic activities, and cellular responses to DNA damage. In drug discovery, it aids in screening compounds targeting OBI1-related pathways. Additionally, it is used to produce antibodies for diagnostic assays, particularly in cancer biomarker studies. Its therapeutic potential is being explored in gene therapy and as a supplement to enhance chemosensitivity in resistant tumors.
The development of OBI1 recombinant protein underscores advancements in precision medicine, offering insights into cancer biology and paving the way for novel treatment strategies. Ongoing research focuses on elucidating its structural motifs, binding partners, and tissue-specific functions to expand its translational relevance.
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