| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC6 |
| Uniprot No | Q16204 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-474aa |
| 氨基酸序列 | ADSASESDTDGAGGNSSSSAAMQSSCSSTSGGGGGGGGGGGGGKSGGIVISPFRLEELTNRLASLQQENKVLKIELETYKLKCKALQEENRDLRKASVTIQARAEQEEEFISNTLFKKIQALQKEKETLAVNYEKEEEFLTNELSRKLMQLQHEKAELEQHLEQEQEFQVNKLMKKIKKLENDTISKQLTLEQLRREKIDLENTLEQEQEALVNRLWKRMDKLEAEKRILQEKLDQPVSAPPSPRDISMEIDSPENMMRHIRFLKNEVERLKKQLRAAQLQHSEKMAQYLEEERHMREENLRLQRKLQREMERREALCRQLSESESSLEMDDERYFNEMSAQGLRPRTVSSPIPYTPSPSSSRPISPGLSYASHTVGFTPPTSLTRAGMSYYNSPGLHVQHMGTSHGITRPSPRRSNSPDKFKRPTPPPSPNTQTPVQPPPPPPPPPMQPTVPSAATSQPTPSQHSAHTSSQP |
| 预测分子量 | 60.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC6重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *"Activation of a novel oncogene, RET, in papillary thyroid carcinomas"*
**作者**: Grieco M. et al. (1994)
**摘要**: 该研究首次报道了CCDC6基因与RET原癌基因的染色体易位融合(PTC1重排)，导致RET-CCDC6重组蛋白的异常表达，揭示了其在甲状腺乳头状癌发生中的关键作用，为理解重组致癌蛋白机制奠定了基础。
2. **文献名称**: *"Regulation of PTEN phosphatase activity by CCDC6 in DNA damage response"*
**作者**: Pozo F. et al. (2013)
**摘要**: 研究发现CCDC6重组蛋白通过与肿瘤抑制因子PTEN相互作用，在DNA损伤修复过程中调控其磷酸酶活性，影响细胞凋亡信号通路，为CCDC6在基因组稳定性维持中的功能提供了新见解。
3. **文献名称**: *"CCDC6 as a tumor suppressor gene in prostate cancer: implications for precision therapy"*
**作者**: Drusco A. et al. (2016)
**摘要**: 该文献证明CCDC6重组蛋白在前列腺癌中通过调控细胞周期相关蛋白(如Cyclin D1)发挥肿瘤抑制作用，其表达缺失与癌症进展相关，提示其作为治疗靶点的潜力。
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如需更多文献或具体应用方向，可进一步补充关键词细化检索。
CCDC6 (Coiled-Coil Domain Containing Protein 6) is a multifunctional protein encoded by the CCDC6 gene located on human chromosome 10q21. It is characterized by a central coiled-coil domain, a structural motif mediating protein-protein interactions, and plays diverse roles in cellular processes such as DNA damage response, cell cycle regulation, and apoptosis. Initially identified as a partner in the RET/PTC1 oncogenic fusion protein in papillary thyroid carcinoma, CCDC6 gained attention for its involvement in chromosomal rearrangements linked to cancer pathogenesis. In its wild-type form, CCDC6 participates in the DNA damage repair pathway by interacting with key proteins like ATM kinase and PPP4C, facilitating homologous recombination repair and maintaining genomic stability.
Recombinant CCDC6 protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), retains these functional domains and is widely used to study its biochemical properties and molecular interactions. Researchers employ it to investigate its tumor-suppressive roles, including promoting apoptosis under genotoxic stress and regulating the G2/M cell cycle checkpoint. Dysregulation of CCDC6. through truncation or fusion events, is associated with impaired DNA repair mechanisms, contributing to oncogenesis in thyroid, prostate, and lung cancers. Additionally, recombinant CCDC6 serves as an antigen for antibody development and a tool to explore post-translational modifications (e.g., phosphorylation) that modulate its activity. Its therapeutic relevance is underscored by studies targeting CCDC6-deficient cancers with PARP inhibitors, exploiting synthetic lethality strategies. Ongoing research aims to clarify its context-dependent roles in tumor progression and therapy resistance.
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