| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | A173T |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | ; |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于A173T重组蛋白的虚构参考文献示例(注：以下内容为模拟创作，非真实文献)：
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1. **文献名称**：*Structural and Functional Analysis of A173T Mutant Recombinant Protein in Neurodegenerative Disease Models*
**作者**：J. Smith et al.
**摘要**：本研究通过X射线晶体学解析了A173T重组蛋白的突变结构，发现该突变导致蛋白活性中心构象改变，显著降低其与底物结合能力。体外细胞实验表明，A173T突变体加剧了神经细胞凋亡，提示其与特定神经退行性疾病的潜在关联。
2. **文献名称**：*A173T Recombinant Protein Alters Cellular Signaling Pathways in Breast Cancer Cells*
**作者**：L. Chen & M. Tanaka
**摘要**：作者通过CRISPR技术构建A173T重组蛋白表达模型，发现该突变激活PI3K/AKT通路，促进乳腺癌细胞增殖和迁移。研究为靶向A173T突变的个性化治疗提供了实验依据。
3. **文献名称**：*In Vivo Effects of A173T Recombinant Protein on Cardiac Function in Transgenic Mice*
**作者**：R. Gupta et al.
**摘要**：利用转基因小鼠模型，研究发现A173T重组蛋白异常表达导致心肌纤维化和收缩功能障碍，提示该突变可能是遗传性心肌病的致病因素之一。蛋白组学分析进一步揭示了线粒体能量代谢相关通路异常。
4. **文献名称**：*Drug Screening for A173T Recombinant Protein Stabilizers Using High-Throughput Assays*
**作者**：K. Müller et al.
**摘要**：针对A173T突变体的热不稳定性，开发高通量筛选平台并筛选出小分子化合物C-27.可恢复突变蛋白的折叠稳定性，为基于蛋白修复的疗法奠定基础。
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以上内容仅为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索。若需真实文献，请提供更具体的蛋白名称或研究背景。
The A173T recombinant protein is a genetically engineered variant of a viral protein, commonly associated with the spike (S) protein of coronaviruses such as SARS-CoV-2. This mutation involves a single amino acid substitution (alanine to threonine) at position 173 within the receptor-binding domain (RBD) or adjacent regions, depending on the specific viral strain. The mutation is of interest due to its potential impact on viral infectivity, immune evasion, or interactions with host cell receptors like ACE2. Recombinant A173T proteins are typically produced using expression systems such as mammalian (HEK293. CHO) or insect cells to ensure proper post-translational modifications, mimicking native protein structure.
Researchers utilize the A173T recombinant protein to study evolutionary adaptations of viruses, assess antibody neutralization efficiency, and evaluate vaccine efficacy against emerging variants. Its characterization often involves techniques like ELISA, surface plasmon resonance (SPR), cryo-EM, or X-ray crystallography to analyze binding affinity, structural stability, and antigenic properties. In therapeutic development, this protein serves as a critical tool for screening monoclonal antibodies or designing next-generation vaccines targeting mutation-prone regions.
The A173T mutation has been observed in certain viral lineages under selective pressure, highlighting its role in host-pathogen coevolution. Studies on such variants contribute to pandemic preparedness by elucidating mechanisms of immune escape and informing public health strategies. Current research focuses on correlating this mutation with transmissibility changes and clinical outcomes, underscoring its relevance in virology and immunology.
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