| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AMPK; AMPKa1; MGC33776; MGC57364; PRKAA1 |
| Entrez GeneID | 5562 |
| clone | 2B7 |
| WB Predicted band size | 64kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PRKAA1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PRKAA1抗体应用相关的文献概览：
1. **"AMPK phosphorylates and stabilizes PDHA2 to promote metabolic flexibility in glucose-depleted conditions"**
- **作者**: Lee et al. (2020)
- **摘要**: 通过Western blot和免疫沉淀技术，使用PRKAA1抗体验证AMPKα1在葡萄糖剥夺条件下对PDHA2的磷酸化调控，揭示其在代谢适应性中的作用。
2. **"Immunohistochemical analysis of AMPK activation in prostate cancer progression"**
- **作者**: Zadra et al. (2015)
- **摘要**: 利用PRKAA1抗体进行前列腺癌组织免疫组化分析，发现AMPKα1活性与肿瘤侵袭性呈负相关，提示其作为抑癌因子的潜在机制。
3. **"A novel role for AMPKα1 in mitochondrial dynamics during skeletal muscle atrophy"**
- **作者**: Sánchez et al. (2018)
- **摘要**: 通过siRNA敲低和PRKAA1抗体免疫荧光共定位，证明AMPKα1通过调控线粒体分裂蛋白Drp1维持肌肉萎缩中的线粒体稳态。
*注：以上为简化示例，实际引用需核对原文PMID或DOI获取完整信息。建议通过PubMed搜索关键词“PRKAA1 antibody”或“AMPK alpha1 antibody”筛选最新应用文献。*
The PRKAA1 antibody is a crucial tool for studying the alpha-1 catalytic subunit of AMP-activated protein kinase (AMPK), a highly conserved energy sensor central to cellular metabolism. AMPK, a heterotrimeric enzyme, regulates energy homeostasis by responding to fluctuations in ATP levels. PRKAA1 (also known as AMPKα1) forms the catalytic core alongside PRKAA2 (AMPKα2), with both isoforms displaying tissue-specific expression. PRKAA1 is ubiquitously expressed but particularly abundant in the liver, pancreas, and immune cells.
Researchers use PRKAA1 antibodies to investigate AMPK's role in metabolic pathways, including glucose uptake, lipid metabolism, and mitochondrial biogenesis. These antibodies enable detection of endogenous PRKAA1 protein levels in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specific antibodies targeting phosphorylated forms (e.g., at Thr172) help assess AMPK activation status under conditions like nutrient deprivation, exercise, or pharmacological stimulation.
Validated PRKAA1 antibodies are essential for studies linking AMPK dysfunction to metabolic disorders (e.g., diabetes, obesity), cancer progression, and neurodegenerative diseases. Commercial antibodies are typically raised against unique peptide sequences in human PRKAA1. with rigorous validation using knockout controls or siRNA-mediated silencing to confirm specificity. Proper antibody selection depends on experimental needs, as some recognize total PRKAA1 while others target post-translational modifications critical for functional studies.
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