| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs; PRKDC |
| Entrez GeneID | 5591 |
| clone | 3H6 |
| WB Predicted band size | 46.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRKDC expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于PRKDC抗体的3篇参考文献示例，内容基于模拟生成：
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1. **文献名称**：*PRKDC expression in non-small cell lung cancer correlates with radiotherapy resistance and serves as a prognostic marker*
**作者**：Chen et al. (2017)
**摘要**：该研究通过免疫组化(使用PRKDC抗体)和Western blot分析，发现非小细胞肺癌中PRKDC蛋白高表达与放疗抵抗及患者生存率降低相关，提示其作为潜在治疗靶点。
2. **文献名称**：*A novel PRKDC mutation identified in a patient with severe combined immunodeficiency using targeted sequencing*
**作者**：Gomez et al. (2020)
**摘要**：研究报道一名严重联合免疫缺陷症患儿的PRKDC基因突变，通过PRKDC抗体进行蛋白质印迹验证，显示突变导致PRKDC蛋白功能丧失，揭示了其在免疫缺陷中的作用。
3. **文献名称**：*DNA-PKcs inhibition sensitizes colorectal cancer cells to cetuximab by impairing AKT signaling*
**作者**：Wang et al. (2019)
**摘要**：利用PRKDC抗体进行免疫沉淀及细胞功能实验，证明抑制DNA-PKcs(PRKDC编码蛋白)可增强结直肠癌细胞对西妥昔单抗的敏感性，机制涉及AKT信号通路调控。
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**注**：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索确认。若需具体文献，建议使用关键词“PRKDC antibody”或“DNA-PKcs”结合研究领域(如癌症、免疫缺陷)进一步筛选。
The PRKDC antibody targets the protein encoded by the PRKDC gene, also known as DNA-dependent protein kinase catalytic subunit (DNA-PKcs). This large serine/threonine kinase is a critical component of the DNA-dependent protein kinase (DNA-PK) complex, which plays a central role in the non-homologous end joining (NHEJ) pathway for repairing DNA double-strand breaks (DSBs). DNA-PKcs binds to broken DNA ends alongside Ku70/Ku80 heterodimers, facilitating the recruitment of repair factors and stabilizing damaged DNA during repair. PRKDC is essential for maintaining genomic stability, V(D)J recombination in immune cells, and telomere maintenance.
Researchers use PRKDC antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study its expression, localization, and interactions in cellular contexts. Dysregulation of PRKDC is linked to cancer progression, as its overexpression in tumors often correlates with resistance to radiation or chemotherapy. Conversely, mutations in PRKDC can result in severe combined immunodeficiency (SCID) due to defective lymphocyte development. PRKDC inhibitors are also explored in oncology to sensitize cancer cells to DNA-damaging therapies. The antibody thus serves as a vital tool for investigating DNA repair mechanisms, immune disorders, and therapeutic strategies targeting genomic instability.
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