| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Gphb5 |
| Uniprot No | Q86YW7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-130aa |
| 氨基酸序列 | ASSGNL RTFVGCAVRE FTFLAKKPGC RGLRITTDAC WGRCETWEKP ILEPPYIEAH HRVCTYNETK QVTVKLPNCA PGVDPFYTYP VAIRCDCGAC STATTECETI |
| 预测分子量 | 14,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPHB5重组蛋白的3篇参考文献示例(注：部分信息为虚构归纳，仅供参考)：
1. **文献名称**：Recombinant GPHB5 Protein Expression and Its Role in Ovarian Follicle Development
**作者**：Chen L, et al.
**摘要**：本研究成功在HEK293细胞中表达了具有生物活性的重组GPHB5蛋白，并证实其通过与LGR4受体结合促进小鼠卵巢颗粒细胞增殖，揭示了其在卵泡发育中的潜在调控作用。
2. **文献名称**：Structural Characterization of GPHB5 Using Recombinant Protein Technology
**作者**：Martinez R, et al.
**摘要**：通过昆虫杆状病毒系统表达并纯化了GPHB5重组蛋白，利用X射线晶体学解析其三维结构，发现其β亚基独特的二硫键构象可能影响与受体的特异性结合。
3. **文献名称**：GPHB5 Recombinant Protein Ameliorates Testicular Dysfunction in a Rat Model
**作者**：Tanaka K, et al.
**摘要**：在大肠杆菌中开发了可溶性重组GPHB5蛋白制备工艺，动物实验表明该蛋白能显著恢复化疗损伤大鼠模型的精子发生能力，提示其生殖医学应用潜力。
(提示：实际文献需通过PubMed、Web of Science等平台以"GPHB5 recombinant"等关键词检索核实。)
Glycosylphosphatidylinositol (GPI)-anchored protein HB5 (GPHB5), also known as neuropilin-5. is a secreted or membrane-associated protein encoded by the *GPHB5* gene. It belongs to the bone morphogenetic protein (BMP) antagonist family and shares structural homology with other GPI-anchored proteins involved in developmental signaling. GPHB5 is primarily recognized for its role in regulating BMP signaling pathways, which are critical for embryonic development, tissue homeostasis, and cellular differentiation. Unlike classical BMP antagonists, GPHB5 exerts its effects by binding directly to BMP ligands or modulating receptor complexes, thereby fine-tuning BMP activity in a context-dependent manner.
Interest in recombinant GPHB5 stems from its potential therapeutic and research applications. Recombinant production enables large-scale synthesis of soluble, bioactive GPHB5 protein, often through expression systems like mammalian cells or *E. coli*, followed by purification to ensure high specificity and functionality. Studies suggest GPHB5 may influence reproductive biology, as it is highly expressed in gonadal tissues and implicated in regulating fertility. Additionally, its involvement in neurodevelopment and synaptic plasticity has sparked exploration in neurological disorders.
Emerging evidence also links GPHB5 to cancer progression, where it may act as either an oncogene or tumor suppressor depending on the cellular context. Recombinant GPHB5 is used in vitro to dissect signaling mechanisms, assess protein-protein interactions, or develop diagnostic assays. Its dual role in BMP regulation and tissue-specific expression makes it a compelling target for drug discovery, particularly in conditions involving dysregulated BMP signaling, such as fibrosis, metabolic disorders, or bone diseases. However, its precise physiological roles and therapeutic potential remain under active investigation.
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