| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 1/200 - 1/1000 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | 60S ribosomal export protein NMD3, hNMD3, NMD3 |
| Entrez GeneID | 51068 |
| WB Predicted band size | 57.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NMD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-440 amino acids from the C-terminal region of human NMD3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACTA2抗体的3篇虚构参考文献示例(注：内容为模拟生成，非真实文献)：
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1. **标题**: *ACTA2 Expression in Vascular Smooth Muscle Cells During Atherosclerosis Progression*
**作者**: Smith J, et al.
**摘要**: 研究通过免疫组化和Western blot分析，利用ACTA2抗体揭示了动脉粥样硬化模型中血管平滑肌细胞(VSMCs)的表型转化，发现ACTA2表达下调与斑块不稳定性相关。
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2. **标题**: *ACTA2 as a Marker for Cancer-Associated Fibroblasts in Colorectal Tumors*
**作者**: Lee H, et al.
**摘要**: 本文使用ACTA2抗体对结直肠癌组织进行染色，证实ACTA2阳性成纤维细胞与肿瘤侵袭性相关，提示其在肿瘤微环境中的促癌作用。
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3. **标题**: *ACTA2 Mutations and Vascular Pathology: Immunohistochemical Analysis*
**作者**: Garcia R, et al.
**摘要**: 通过ACTA2抗体对遗传性主动脉疾病患者的组织分析，发现特定突变导致ACTA2蛋白异常聚集，为临床诊断提供了分子病理学依据。
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4. **标题**: *Role of ACTA2-Positive Myofibroblasts in Liver Fibrosis*
**作者**: Chen L, et al.
**摘要**: 研究利用ACTA2抗体鉴定肝纤维化中的肌成纤维细胞来源，证实其与胶原沉积程度正相关，为抗纤维化治疗提供靶点。
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(注：以上作者及文献名为虚构，实际文献需通过PubMed等数据库检索。)
ACTA2 (Alpha-smooth muscle actin) antibodies are essential tools in biomedical research and diagnostics, targeting the protein encoded by the *ACTA2* gene. This gene produces α-smooth muscle actin (α-SMA), a cytoskeletal protein predominantly expressed in vascular and visceral smooth muscle cells, as well as myofibroblasts. α-SMA is critical for maintaining cell structure, motility, and contractile functions, playing key roles in processes like wound healing, tissue fibrosis, and vascular tone regulation.
ACTA2 antibodies are widely used to identify smooth muscle cells and myofibroblasts in tissues, aiding in the study of diseases involving abnormal smooth muscle proliferation or fibrosis, such as atherosclerosis, cancer stroma formation, and organ fibrosis (e.g., liver cirrhosis, pulmonary fibrosis). In cancer research, these antibodies help characterize tumor microenvironments by labeling cancer-associated fibroblasts. Additionally, they are employed in vascular pathology to assess smooth muscle cell differentiation in arterial remodeling or aneurysms.
Mutations in the *ACTA2* gene are linked to hereditary thoracic aortic aneurysms and dissections (TAAD), making these antibodies valuable for investigating genetic vascular disorders. In diagnostics, ACTA2 immunohistochemistry assists in differentiating tumors of smooth muscle origin (e.g., leiomyomas) from other mesenchymal neoplasms.
Commercially available ACTA2 antibodies are typically validated for techniques like immunohistochemistry, Western blotting, and immunofluorescence. Their specificity ensures reliable detection of α-SMA in formalin-fixed tissues or cultured cells, though cross-reactivity with other actin isoforms requires careful experimental controls.
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