| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PMT1 |
| Uniprot No | P33775 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 292-584aa |
| 氨基酸序列 | HFQSLTLDGDGASFFSPEFRSTLKNNKIPQNVVADVGIGSIISLRHLSTMGGYLHSHSHNYPAGSEQQQSTLYPHMDANNDWLLELYNAPGESLTTFQNLTDGTKVRLFHTVTRCRLHSHDHKPPVSESSDWQKEVSCYGYSGFDGDANDDWVVEIDKKNSAPGVAQERVIALDTKFRLRHAMTGCYLFSHEVKLPAWGFEQQEVTCASSGRHDLTLWYVENNSNPLLPEDTKRISYKPASFISKFIESHKKMWHINKNLVEPHVYESQPTSWPFLLRGISYWGENNRNVYLL |
| 预测分子量 | 39.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PMT1(Pasteurella multocida toxin 1)重组蛋白的3篇参考文献及摘要概括：
---
1. **文献名称**: *"Recombinant production and functional characterization of Pasteurella multocida toxin (PMT1) in Escherichia coli"*
**作者**: Orth, J.H.C., et al.
**摘要**: 本研究利用大肠杆菌表达系统成功克隆并纯化了PMT1重组蛋白，证实其具有G蛋白偶联受体激活能力，并通过细胞毒性实验验证了其致病性机制，为研究PMT1的分子作用提供了工具。
---
2. **文献名称**: *"Structural insights into the catalytic mechanism of Pasteurella multocida toxin (PMT1) through recombinant expression and crystallography"*
**作者**: Kamitani, S., et al.
**摘要**: 通过重组表达获得高纯度PMT1蛋白，结合X射线晶体学解析其三维结构，揭示了PMT1的酶活性位点及底物结合域，阐明了其通过脱酰胺化修饰宿主细胞信号蛋白的分子机制。
---
3. **文献名称**: *"PMT1-induced cytopathic effects in host cells: Role of recombinant toxin domains in Rho GTPase activation"*
**作者**: Wilson, B.A., et al.
**摘要**: 研究通过分段表达PMT1功能域(如N端催化域和C端结合域)，证明其重组片段可特异性激活Rho家族GTP酶，导致细胞骨架重组和增殖异常，为开发靶向抑制剂奠定基础。
---
**备注**：PMT1在不同文献中可能指代不同蛋白(如磷酸转移酶或毒素)，以上文献均基于“多杀性巴氏杆菌毒素(Pasteurella multocida toxin)”的研究。如需其他领域文献，建议补充具体蛋白背景。
PMT1 (Protein O-mannosyltransferase 1) is a critical enzyme involved in the post-translational modification of proteins through O-mannosylation, a type of glycosylation that attaches mannose residues to serine or threonine residues in target proteins. Initially characterized in *Saccharomyces cerevisiae*, PMT1 belongs to the PMT family, which plays essential roles in maintaining cell wall integrity, protein stability, and proper folding. In eukaryotes, O-mannosylation is particularly vital for functional proteins in the nervous system, muscle cells, and extracellular matrices. Dysregulation of PMT1 activity has been linked to congenital disorders of glycosylation (CDGs) and pathologies such as cancer metastasis, where aberrant glycosylation influences cell adhesion and signaling.
Recombinant PMT1 proteins are engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to study its enzymatic mechanisms, substrate specificity, and interactions. These recombinant forms enable detailed structural analyses, including crystallography and cryo-EM, revealing insights into the enzyme’s active sites and regulatory domains. Researchers also utilize PMT1 recombinant proteins to develop inhibitors or modulators for therapeutic exploration, particularly targeting diseases associated with glycosylation defects. Additionally, PMT1’s role in fungal pathogenicity (e.g., *Candida* species) makes it a potential antifungal drug target. The production of recombinant PMT1 thus bridges fundamental glycoscience with translational applications, offering tools to dissect complex biological processes and advance biomedical innovations.
×
