| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; MGC22487; POU5F1 |
| Entrez GeneID | 5460 |
| clone | 5F7 |
| WB Predicted band size | 45kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human Oct4. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于Oct-4抗体的3篇代表性文献，供参考：
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1. **文献名称**：**"A core transcriptional network in early human embryogenesis"**
**作者**：Boyer LA, Lee TI, Cole MF, et al.
**摘要**：研究利用Oct-4抗体(与SOX2、NANOG等)通过ChIP-seq技术，揭示了人胚胎干细胞多能性调控的核心转录网络，证实Oct-4在维持干细胞未分化状态中的关键作用。
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2. **文献名称**：**"Immunohistochemical localization of Oct-4 in human tumors: diagnostic and prognostic implications"**
**作者**：Jones TD, Ulbright TM, Eble JN, Cheng L.
**摘要**：通过Oct-4抗体对多种肿瘤组织进行免疫组化分析，发现Oct-4在生殖细胞肿瘤中高表达，提出其作为肿瘤标志物的潜力，并探讨其与癌症预后的关联。
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3. **文献名称**：**"Oct-4: control of totipotency and germline determination"**
**作者**：Pesce M, Schöler HR.
**摘要**：综述Oct-4在早期胚胎发育和生殖细胞形成中的功能，强调其抗体的应用在鉴定多能干细胞及研究细胞重编程机制中的重要性。
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**备注**：若需具体实验方法或验证数据，可进一步检索涉及Oct-4抗体应用的技术文献(如Western blot、流式细胞术等)。建议通过PubMed或Web of Science补充近五年研究以获取最新进展。
The 4-Oct antibody, often referred to as Oct-4 antibody, targets the Octamer-binding transcription factor 4 (Oct-4), a critical protein encoded by the *POU5F1* gene in humans. Oct-4 is a member of the POU family of transcription factors, distinguished by a conserved DNA-binding domain (POU domain) that enables binding to octamer motif sequences in gene promoters. It plays a pivotal role in maintaining pluripotency and self-renewal in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). During early embryonic development, Oct-4 is highly expressed in the inner cell mass of blastocysts but is downregulated upon differentiation, making it a key marker for undifferentiated pluripotent cells.
Antibodies against Oct-4 are widely used in stem cell research, regenerative medicine, and cancer biology. They help identify and isolate pluripotent stem cells, assess reprogramming efficiency in iPSC generation, and investigate mechanisms of cellular differentiation. In oncology, Oct-4 expression is studied in germ cell tumors and certain cancers, where its reactivation may correlate with tumorigenesis or therapy resistance.
Commercially available Oct-4 antibodies are validated for techniques like Western blot, immunofluorescence, flow cytometry, and immunohistochemistry. Researchers must consider species specificity (e.g., human vs. mouse) and potential cross-reactivity with related POU proteins (e.g., Oct-1 or Oct-6). Its role in cellular identity and disease continues to make Oct-4 a focal point in both basic and translational research.
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