| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AK1 |
| Uniprot No | P00568 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-194aa |
| 氨基酸序列 | MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK |
| 预测分子量 | 37.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AK1重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括列出：
1. **文献名称**：*"High-yield production and characterization of recombinant human adenylate kinase 1 (AK1) in Escherichia coli"*
**作者**：Smith J, et al.
**摘要**：该研究报道了人源AK1在大肠杆菌中的高效重组表达与纯化方法，通过优化表达条件及亲和层析技术获得高纯度蛋白，并验证其酶活性，为后续功能研究奠定基础。
2. **文献名称**：*"Structural insights into the catalytic mechanism of AK1: Implications for metabolic regulation"*
**作者**：Chen L, Wang H, et al.
**摘要**：利用X射线晶体学解析了重组AK1的三维结构，揭示了其ATP/AMP结合位点的关键氨基酸残基，阐明了AK1在能量代谢中的催化机制及潜在调控位点。
3. **文献名称**：*"Recombinant AK1 attenuates ischemic injury in cardiomyocytes via AMPK signaling pathway"*
**作者**：Zhang Y, et al.
**摘要**：研究证明外源性重组AK1可通过激活AMPK通路减少心肌细胞缺血损伤，提示其作为治疗能量代谢相关心脏疾病的潜在靶点。
(注：以上文献为虚拟示例，实际引用需根据具体研究检索真实数据库。)
**Background of AK1 Recombinant Protein**
Adenylate kinase 1 (AK1), a ubiquitously expressed enzyme, plays a critical role in cellular energy homeostasis by catalyzing the reversible transfer of a phosphate group between adenosine nucleotides (ATP + AMP ↔ 2 ADP). This reaction ensures rapid regeneration of ATP, maintaining energy balance essential for processes like muscle contraction, ion transport, and signal transduction. AK1 is predominantly localized in the cytosol and mitochondrial intermembrane space, with elevated expression in tissues with high metabolic demands, such as the brain, heart, and skeletal muscle.
The human *AK1* gene, located on chromosome 9. encodes a ~22 kDa protein comprising 194 amino acids. Its structure features a conserved catalytic core with distinct nucleotide-binding domains (NBD) and a flexible LID region, enabling substrate recognition and conformational changes during catalysis. Dysregulation of AK1 has been linked to metabolic disorders, mitochondrial dysfunction, and certain cancers, highlighting its physiological and pathological relevance.
Recombinant AK1 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), ensuring high purity and activity for research applications. It serves as a vital tool for studying enzyme kinetics, nucleotide metabolism, and energy-sensing pathways like AMPK. Additionally, AK1 is utilized in drug discovery to screen inhibitors or modulators targeting metabolic diseases. Its stability and conserved structure across species also make it a model for evolutionary and structural biology studies.
In summary, AK1 recombinant protein bridges fundamental biochemical research with translational applications, offering insights into cellular energetics and therapeutic strategies for metabolic impairments.
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