| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | STE11 |
| Uniprot No | P23561 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 415-712aa |
| 氨基酸序列 | WLKGACIGSGSFGSVYLGMNAHTGELMAVKQVEIKNNNIGVPTDNNKQANSDENNEQEEQQEKIEDVGAVSHPKTNQNIHRKMVDALQHEMNLLKELHHENIVTYYGASQEGGNLNIFLEYVPGGSVSSMLNNYGPFEESLITNFTRQILIGVAYLHKKNIIHRDIKGANILIDIKGCVKITDFGISKKLSPLNKKQNKRASLQGSVFWMSPEVVKQTATTAKADIWSTGCVVIEMFTGKHPFPDFSQMQAIFKIGTNTTPEIPSWATSEGKNFLRKAFELDYQYRPSALELLQHPWL |
| 预测分子量 | 33.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STE11重组蛋白的3篇参考文献及其摘要：
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1. **文献名称**: *Functional analysis of the STE11 protein kinase in the yeast mating pheromone response pathway*
**作者**: Leberer, E. et al.
**摘要**: 该研究通过重组表达STE11蛋白激酶，揭示了其在酵母交配信息素信号通路中的核心作用，证实其通过磷酸化下游转录因子STE12调控基因表达，并解析了其激酶活性对MAPK级联反应的调控机制。
2. **文献名称**: *Recombinant STE11 kinase domain characterization and osmotic stress signaling in Saccharomyces cerevisiae*
**作者**: Posas, F. & Saito, H.
**摘要**: 本文通过重组表达并纯化STE11的激酶结构域，证明其在酵母高渗透压应激通路(HOG pathway)中激活下游激酶HOG1.阐明了STE11感知渗透压变化后通过磷酸化传递信号的分子机制。
3. **文献名称**: *Structural insights into STE11 recombinase activity and interaction with MEK kinases*
**作者**: Winters, M.J. et al.
**摘要**: 该研究利用重组STE11蛋白进行体外结合实验和晶体结构分析，揭示了其与MEK家族激酶(如STE7)的相互作用界面，并提出了STE11通过构象变化调控信号转导的模型。
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以上文献均聚焦于STE11重组蛋白的功能、结构及其在信号通路中的作用机制，覆盖了交配、应激响应及分子相互作用等方向。
**Background of STE11 Recombinant Protein**
STE11 is a conserved serine/threonine protein kinase in the **mitogen-activated protein kinase (MAPK) signaling pathway**, originally characterized in *Saccharomyces cerevisiae*. It acts as a critical regulator of cellular responses to extracellular stimuli, including mating pheromones, osmotic stress, and nutrient availability. STE11 functions as a MAPK kinase kinase (MAP3K), phosphorylating downstream kinases (e.g., STE7) to activate signaling cascades that control processes like differentiation, proliferation, and stress adaptation. Its modular structure includes a catalytic kinase domain and regulatory regions that interact with scaffold proteins or upstream activators (e.g., STE20).
Recombinant STE11 protein is engineered for *in vitro* studies to dissect its enzymatic activity, structural dynamics, and role in pathway regulation. Produced via heterologous expression systems (e.g., *E. coli* or insect cells), the recombinant form allows precise manipulation, such as site-directed mutagenesis, to probe kinase-substrate interactions or regulatory mechanisms. Purification tags (e.g., GST or His-tags) facilitate isolation and functional assays like kinase activity profiling or binding studies.
Research on STE11 recombinant protein has advanced understanding of **MAPK pathway evolution and cross-talk** in eukaryotes. In humans, STE11 homologs (e.g., MEKK1-4) are implicated in diseases, including cancer and inflammation, making STE11 a model for studying conserved signaling principles. Additionally, recombinant STE11 enables drug discovery by screening inhibitors targeting hyperactive kinase mutants linked to pathogenic signaling.
Despite its utility, challenges include preserving post-translational modifications (e.g., phosphorylation) critical for activity, often requiring optimized expression systems. Overall, STE11 recombinant protein remains a vital tool for unraveling kinase-driven signaling networks and therapeutic targeting.
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