| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF8 |
| Uniprot No | O76064 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-485aa |
| 氨基酸序列 | MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQL VSKICPLMISRNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSI HQGDYIQLGVPLENKENAEYEYEVTEEDWETIYPCLSPKNDQMIEKNKEL RTKRKFSLDELAGPGAEGPSNLKSKINKVSCESGQPVKSQGKGEVASTPS DNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSSASQRSLQMFK VTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSKKVVQMEQELQDLQSQLC AEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWAL MEELNRSKKDFEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENE LQCIICSEYFIEAVTLNCAHSFCSYCINEWMKRKIECPICRKDIKSKTYS LVLDNCINKMVNNLSSEVKERRIVLIRERKAKRLF |
| 预测分子量 | 79 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF8重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：RNF8 Ubiquitylates Histones at DNA Double-Strand Breaks and Promotes Assembly of Repair Proteins
**作者**：Mailand, N. et al.
**摘要**：该研究揭示了RNF8作为E3泛素连接酶，在DNA双链断裂(DSB)修复中通过泛素化组蛋白H2A，招募BRCA1和53BP1等修复蛋白至损伤位点，依赖其泛素结合结构域(UIM)和重组蛋白实验验证其催化活性。
2. **文献名称**：RNF8 Transduces the DNA-Damage Signal via Catalyzing Ubiquitination of γ-H2AX
**作者**：Huen, M.S.Y. et al.
**摘要**：研究发现RNF8通过催化γ-H2AX的K63泛素链形成，介导DNA损伤信号传导。利用重组RNF8蛋白体外实验，证实其与E2酶UBC13协作，促进修复复合体形成，并依赖FHA结构域识别磷酸化靶标。
3. **文献名称**：Coordinated Protein Kinase and Ubiquitin Ligase Activities Govern the DNA Damage Response
**作者**：Kolas, N.K. et al.
**摘要**：该研究阐明RNF8与RNF168协同调控DNA损伤应答的机制。重组RNF8蛋白实验表明其通过泛素化染色质，招募RNF168扩大泛素信号，从而激活下游修复通路，确保基因组稳定性。
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以上文献聚焦RNF8在DNA修复中的分子机制，均涉及重组蛋白的功能验证(如泛素化活性、蛋白互作)，为理解其生物学作用提供了关键证据。如需具体发表年份或期刊，可进一步补充检索PubMed或SciHub获取全文。
RNF8 (Ring Finger Protein 8) is a ubiquitin E3 ligase critical for maintaining genomic stability and orchestrating DNA damage response (DDR) pathways. Discovered in the early 2000s, RNF8 plays a pivotal role in the signaling cascade triggered by double-strand breaks (DSBs), where it facilitates the recruitment of downstream repair proteins by catalyzing ubiquitination events. Structurally, RNF8 contains an N-terminal forkhead-associated (FHA) domain that binds phosphorylated targets and a C-terminal RING domain responsible for its E3 ligase activity. Upon DSB induction, ATM kinase phosphorylates histone H2AX (γ-H2AX), creating docking sites for mediator proteins like MDC1. RNF8. in complex with the E2 enzyme UBC13. is recruited to these sites and initiates Lys63-linked ubiquitination of histones and surrounding substrates, forming a signaling platform for repair factors such as BRCA1. 53BP1. and RNF168.
Recombinant RNF8 protein is engineered for in vitro and cellular studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains enzymatic activity and interaction capabilities, enabling researchers to study ubiquitination kinetics, protein-protein interactions, and structural features. Its applications span cancer research, particularly in understanding resistance to radiotherapy or chemotherapy, as RNF8 dysfunction correlates with genomic instability and tumorigenesis. Additionally, recombinant RNF8 aids in drug discovery, serving as a target for small molecules aiming to modulate DDR pathways. Despite progress, challenges remain in elucidating context-dependent roles—RNF8 can act as both a tumor suppressor and promoter depending on cellular conditions—highlighting the need for further mechanistic studies using purified recombinant tools.
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