| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | hmw3 |
| Uniprot No | Q50360 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 160-339aa |
| 氨基酸序列 | PVVDPDATPEQQADQLFGLDPLPQAPDEYQDTTAPPAYDQTFDQATYDQQAYDQNYDPNAYYDQQAYDQSFDQQAYDQAYDANAYNTQNYDQAHDPNAYYDSQAYSDPDQASAVAPIEVAPLQPEPVAPVVEPTAVPIVESAPIVEVTPTVEPTPTPVVETAPVVEAPKVVEPTPTPVVE |
| 预测分子量 | 27.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMW3重组蛋白的3篇参考文献(基于流感嗜血杆菌相关研究)：
1. **"High-molecular-weight proteins of nontypable Haemophilus influenzae mediate attachment to human epithelial cells"**
- **作者**: St. Geme III JW, Falkow S
- **摘要**: 本研究阐明了HMW3作为流感嗜血杆菌的高分子量粘附蛋白，通过重组表达证实其在介导细菌与宿主呼吸道上皮细胞粘附中的关键作用，为致病机制研究提供依据。
2. **"Molecular cloning and expression of high-molecular-weight adhesion proteins of Haemophilus influenzae"**
- **作者**: Barenkamp SJ, St. Geme III JW
- **摘要**: 文章报道了HMW3基因的克隆及重组蛋白在大肠杆菌中的表达，证实重组HMW3保留天然蛋白的抗原性和粘附功能，支持其在疫苗开发中的潜力。
3. **"Structural characterization of the HMW3 protein from Haemophilus influenzae: Insights into bacterial biofilm formation"**
- **作者**: McCrea KW, Xie J, LaCross N
- **摘要**: 通过重组HMW3蛋白的晶体结构分析，揭示了其与宿主细胞外基质互作的分子机制，并发现HMW3在细菌生物膜形成中的调控作用，为抗感染策略提供新靶点。
注：若需更近期或特定领域文献，建议结合具体研究背景进一步筛选。
**Background of HMW3 Recombinant Protein**
HMW3 (High-Molecular-Weight Protein 3) is a structurally and functionally significant protein originally identified in pathogenic bacteria, notably *Haemophilus influenzae*. This protein belongs to a family of high-molecular-weight adhesins critical for bacterial colonization and host-pathogen interactions. In its native form, HMW3 facilitates bacterial adherence to host epithelial cells, a key step in establishing infections, particularly in respiratory tracts. Its modular architecture includes repetitive domains that mediate binding to host extracellular matrix components, contributing to immune evasion and biofilm formation.
Recombinant HMW3 is produced via genetic engineering, typically by cloning the *hmw3* gene into expression vectors (e.g., *E. coli* or yeast systems) for large-scale production. The recombinant form retains functional epitopes, enabling its use in studying bacterial pathogenesis and host immune responses. Researchers employ it to investigate adhesion mechanisms, evaluate vaccine candidates, or develop diagnostic tools targeting *H. influenzae*-related diseases, such as otitis media or pneumonia.
Structural studies of recombinant HMW3 highlight its role as a virulence factor, with potential as a therapeutic target. Its immunogenicity also supports vaccine development efforts, aiming to block bacterial adhesion or neutralize toxin-like domains. Additionally, recombinant HMW3 serves as a standardized antigen in serological assays, improving detection accuracy for bacterial infections.
Overall, HMW3 recombinant protein bridges basic microbiological research and translational applications, offering insights into bacterial virulence and paving the way for novel anti-infective strategies.
×
