| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Mindy2 |
| Uniprot No | Q6PDI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601aa |
| 氨基酸序列 | MENSPDSPQPLELGVAAGRVSPPEGRRRGGREAEDGPAGRAVDSGGQGAAAAAARSSLGDPTSPSQLGCGAGSDLKDGASSSPAASEVPSRGQHKVTASPELAEAAAGRGSGPVGDTGTCRVEQAAEEPSSTGAPSSSCSEPSPPGDSPSLDSLESFSNLHSFPSSSEFNSEEGAETRVPEDVEEGAAGPPRAAPLCKEEEEDPAQVLAASKERFPGQSVYHIKWIQWKEENTPIITQNENGPCPLLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLEAKPKEISEIQRVNYEQNMSDAMAILHKLQTGLDVNVRFTGVRVFEYTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSQLVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKIVWESLHNVDGDGNFCDSEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKLQEEEDRRASQYYQEQEQAQAVVTTTTPSTQAQQGQPAQASPSSIKQPGNSERKRKEPREKDKEKEKEKNSCVIL |
| 预测分子量 | 93.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Mindy2重组蛋白的3篇参考文献示例(注：文献信息为虚构，仅供格式参考)：
1. **文献名称**：*"Mindy2 as a Deubiquitinating Enzyme: Structural and Functional Characterization"*
**作者**：Zhang, Y. et al.
**摘要**：本研究解析了重组Mindy2蛋白的晶体结构，揭示了其作为去泛素化酶(DUB)的催化机制，证明其对K48-linked泛素链的特异性识别及水解活性。
2. **文献名称**：*"Regulation of Cell Cycle by Mindy2 through Cyclin D1 Stabilization"*
**作者**：Lee, J. & Kim, S.
**摘要**：通过体外重组Mindy2蛋白实验，发现其通过去泛素化并稳定Cyclin D1.促进G1/S期转换，揭示了Mindy2在细胞周期调控中的作用。
3. **文献名称**：*"Mindy2 Knockout and Recombinant Rescue in Neurodegenerative Models"*
**作者**：Smith, T. et al.
**摘要**：利用重组Mindy2蛋白恢复神经细胞功能，证明其通过调控Tau蛋白泛素化水平，缓解阿尔茨海默病模型中的病理表型。
如需真实文献，建议通过PubMed或Google Scholar检索 **"Mindy2 deubiquitinase"** 或 **"FAM63B recombinant protein"**(Mindy2别名)。
**Background of Mindy2 Recombinant Protein**
Mindy2 (MINDY Lysine 48 Deubiquitinase 2), also known as FAM63B, is a member of the MINDY family of deubiquitinating enzymes (DUBs) that specifically cleave lysine 48 (K48)-linked polyubiquitin chains. These chains typically tag proteins for proteasomal degradation, positioning Mindy2 as a critical regulator of protein stability and turnover. Discovered in 2015. Mindy2 has garnered attention for its role in modulating cellular processes such as DNA damage response, cell cycle progression, and stress signaling. Unlike many DUBs, Mindy2 exhibits unique substrate selectivity, acting on longer K48 chains to rescue targeted proteins from degradation, thereby influencing pathways linked to cancer, neurodegeneration, and immune regulation.
Recombinant Mindy2 protein is engineered for in vitro studies to dissect its enzymatic activity, structure, and interactions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains catalytic residues (Cys/His boxes) essential for DUB function. Researchers utilize purified Mindy2 to explore its substrate specificity, screen inhibitors, or study its role in diseases. For instance, dysregulation of Mindy2 has been implicated in tumorigenesis, where it stabilizes oncoproteins like HIF-1α or Cyclin D1. suggesting therapeutic potential.
Current research also focuses on structural characterization (e.g., X-ray crystallography) to elucidate mechanisms underlying its preference for K48 chains. As a tool, recombinant Mindy2 aids in unraveling ubiquitin code complexity and developing targeted therapies. Its study bridges gaps in understanding post-translational regulation, offering insights into cellular homeostasis and disease pathogenesis.
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