| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIMAP6 |
| Uniprot No | Q6P9H5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEEEEYE QIPQENPPEE LSQDPVLELS GGLREKEQKT PRRLRLILMG KTGSGKSATG NSILGRDVFE SKLSTRPVTK TSQRRSREWA GKELEVIDTP NILSPQVSPE VADAICQAIV LSAPGPHAVL LVTQLGRFTD EDQQVVRRLQ EVFGVGVLGH TILVFTRKED LAGGSLEDYV RETNNQALAW LDVTLARRHC GFNNRAQGEE QEAQLRELME KVEAIMWENE GDYYSNKAYQ YTQQNFRLKE LQERQVSQGQ GSEDVPGEES WLEGLSQIQK ESEEAHRCLL GKADL |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GIMAP6重组蛋白的3篇参考文献摘要概括(基于公开信息模拟，实际文献可能存在差异)：
1. **文献名称**: "GIMAP6 regulates mitochondrial integrity and T cell survival through interaction with the adenine nucleotide translocase"
**作者**: Chen, Y., et al.
**摘要**: 该研究利用重组GIMAP6蛋白进行体外结合实验，发现其通过与线粒体内膜腺苷酸转运蛋白(ANT)相互作用，调控线粒体膜电位稳定性，进而影响T细胞凋亡。实验表明，重组GIMAP6的过表达可抑制ROS积累并增强T细胞存活能力。
2. **文献名称**: "Recombinant GIMAP6 exhibits GTPase-dependent autophagic flux modulation in immune cells"
**作者**: Kumar, S., & Smith, T.J.
**摘要**: 研究团队通过大肠杆菌系统成功表达并纯化具有活性的重组GIMAP6蛋白，证实其具备GTP酶活性。体外细胞实验显示，该蛋白通过激活自噬相关通路(如LC3-II转化)，参与清除受损细胞器，提示其在免疫细胞稳态中的调控作用。
3. **文献名称**: "Structural and functional characterization of human GIMAP6 recombinant protein using X-ray crystallography"
**作者**: Lee, H., et al.
**摘要**: 首次报道了人源GIMAP6重组蛋白的晶体结构(分辨率2.8Å)，揭示其保守的GTP结合口袋及C端螺旋结构域。功能实验表明，重组蛋白的GTP结合能力缺失突变体(K52A)显著降低对Jurkat细胞凋亡的抑制作用，证实其酶活性与抗凋亡功能直接相关。
注：以上内容为领域知识模拟示例，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"GIMAP6 recombinant"或"GIMAP6 protein function"查找最新实证研究。
**Background of GIMAP6 Recombinant Protein**
GIMAP6 (GTPase of the immunity-associated protein family 6) belongs to the GIMAP family, a group of GTPases predominantly expressed in immune cells, particularly lymphocytes. These proteins are evolutionarily conserved and play critical roles in regulating immune cell survival, homeostasis, and function. GIMAP6 is primarily associated with T-cell development and maintenance, where it modulates apoptosis and autophagy processes. Studies suggest its involvement in maintaining mitochondrial integrity and influencing signaling pathways linked to cell survival, though its exact molecular mechanisms remain partially characterized.
The interest in recombinant GIMAP6 stems from its potential role in immune-related disorders. Dysregulation of GIMAP6 has been implicated in autoimmune diseases (e.g., type 1 diabetes, rheumatoid arthritis) and immunodeficiency conditions. Recombinant GIMAP6 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables researchers to study its biochemical properties, interactions, and functional pathways *in vitro*. This protein is often engineered with tags (e.g., His-tag) for purification and detection, ensuring high purity and activity for experimental use.
Structurally, GIMAP6 contains a conserved GTP-binding domain and a C-terminal coiled-coil motif, which may facilitate protein-protein interactions. Recombinant versions retain these features, allowing investigations into GTPase activity, oligomerization, or binding partners. Current research leverages GIMAP6 recombinant protein to explore its therapeutic potential, such as targeting its activity to modulate immune cell survival in cancer or autoimmune contexts. Additionally, it serves as a tool for diagnostic development, including antibody detection assays in autoimmune profiling.
Despite progress, gaps persist in understanding its tissue-specific roles and regulatory networks, highlighting the need for further structural and functional studies using recombinant GIMAP6 as a key reagent.
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