| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SRP72 |
| Uniprot No | O76094 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-671aa |
| 氨基酸序列 | ASGGSGGVSVPALWSEVNRYGQNGDFTRALKTVNKILQINKDDVTALHCKVVCLIQNGSFKEALNVINTHTKVLANNSLSFEKAYCEYRLNRIENALKTIESANQQTDKLKELYGQVLYRLERYDECLAVYRDLVRNSQDDYDEERKTNLSAVVAAQSNWEKVVPENLGLQEGTHELCYNTACALIGQGQLNQAMKILQKAEDLCRRSLSEDTDGTEEDPQAELAIIHGQMAYILQLQGRTEEALQLYNQIIKLKPTDVGLLAVIANNIITINKDQNVFDSKKKVKLTNAEGVEFKLSKKQLQAIEFNKALLAMYTNQAEQCRKISASLQSQSPEHLLPVLIQAAQLCREKQHTKAIELLQEFSDQHPENAAEIKLTMAQLKISQGNISKACLILRSIEELKHKPGMVSALVTMYSHEEDIDSAIEVFTQAIQWYQNHQPKSPAHLSLIREAANFKLKYGRKKEAISDLQQLWKQNPKDIHTLAQLISAYSLVDPEKAKALSKHLPSSDSMSLKVDVEALENSAGATYIRKKGGKVTGDSQPKEQGQGDLKKKKKKKKGKLPKNYDPKVTPDPERWLPMRERSYYRGRKKGKKKDQIGKGTQGATAGASSELDASKTVSSPPTSPRPGSAATVSASTSNIIPPRHQKPAGAPATKKKQQQKKKKGGKGGW |
| 预测分子量 | 101.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于SRP72重组蛋白的假设参考文献示例(基于相关领域研究方向的概括，供参考)：
1. **"Functional characterization of recombinant SRP72 in mammalian protein translocation"**
- 作者：Zhang Y. et al.
- 摘要：研究通过重组表达人源SRP72蛋白，验证其在信号识别颗粒(SRP)复合体中的核心作用，揭示其与核糖体及新生肽链的相互作用机制。
2. **"Structural insights into SRP72 architecture using recombinant protein crystallography"**
- 作者：Smith J.R. & Patel T.
- 摘要：利用重组SRP72蛋白进行X射线晶体学分析，解析其N端结构域的三维构象，阐明其在SRP组装中的关键结合界面。
3. **"Disease-associated mutations in SRP72 disrupt recombinant protein assembly in vitro"**
- 作者：Brown K.A. et al.
- 摘要：通过体外重组实验，证明骨髓衰竭综合征相关的SRP72突变会破坏其与SRP68的互作，导致SRP复合体功能异常。
4. **"Optimization of recombinant SRP72 expression in E. coli for functional studies"**
- 作者：Lee S. & Kim M.
- 摘要：报道一种高效的大肠杆菌表达系统，用于可溶性SRP72重组蛋白的大规模制备，支持后续生化与药物筛选研究。
(注：以上为模拟文献，实际文献需通过PubMed/Google Scholar等平台以“SRP72 recombinant”等关键词检索获取。)
**Background of SRP72 Recombinant Protein**
The SRP72 (Signal Recognition Particle 72 kDa) protein is a critical component of the signal recognition particle (SRP), a ribonucleoprotein complex essential for co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP72. along with SRP68. forms the protein heterodimer that binds directly to the SRP RNA, stabilizing the complex and mediating its interaction with the ribosome. This interaction ensures proper delivery of nascent polypeptide chains to the ER membrane via the SRP receptor, facilitating protein translocation or membrane integration.
Recombinant SRP72 is produced using biotechnological methods, such as expression in bacterial (e.g., *E. coli*) or mammalian cell systems, followed by purification to homogeneity. Its recombinant form enables detailed structural and functional studies, overcoming challenges associated with isolating the native protein from cellular environments. Researchers utilize SRP72 recombinant protein to investigate its role in SRP assembly, RNA binding, and its contribution to diseases linked to SRP dysfunction. For instance, mutations in *SRP72* have been associated with congenital neutropenia and bone marrow failure syndromes, highlighting its importance in hematopoiesis.
Additionally, recombinant SRP72 serves as a tool for exploring interactions with other SRP subunits, ribosomes, or therapeutic molecules. It aids in deciphering molecular mechanisms underlying SRP-related pathologies and screening potential drugs targeting SRP-mediated protein biogenesis. Studies leveraging this recombinant protein also contribute to understanding evolutionary conservation of the SRP pathway across species. Overall, SRP72 recombinant protein is pivotal for advancing both basic research and translational applications in cell biology and genetic disorders.
×
