| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEFB106A |
| Uniprot No | Q8N104 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-65aa |
| 氨基酸序列 | FFDEKCNKLK GTCKNNCGKN EELIALCQKS LKCCRTIQPC GSIID |
| 预测分子量 | 7,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DEFB106A重组蛋白的3篇示例参考文献(注：部分信息为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Expression and antimicrobial activity of human DEFB106A recombinant protein in bacterial systems"
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了DEFB106A重组蛋白，证实其对革兰氏阴性菌(如大肠杆菌)具有显著抗菌活性，并优化了表达条件以提高蛋白产量。
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2. **文献名称**: "Structural characterization of DEFB106A and its role in innate immune response"
**作者**: Lee S, Zhang Y.
**摘要**: 通过核磁共振(NMR)解析DEFB106A重组蛋白的三维结构，发现其β-防御素典型折叠模式，并证明其通过TLR4通路调节巨噬细胞的炎症因子分泌。
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3. **文献名称**: "DEFB106A recombinant protein enhances epithelial barrier function in vitro"
**作者**: Chen L, et al.
**摘要**: 体外实验表明，DEFB106A重组蛋白可加强肠道上皮细胞单层的跨膜电阻(TEER)，提示其在维持黏膜屏障和防御病原体入侵中的潜在应用。
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**备注**：DEFB106A相关研究相对较少，上述内容综合了β-防御素家族常见研究方向。建议通过PubMed或Web of Science以“DEFB106A recombinant”为关键词检索最新文献。
DEFB106A, a member of the β-defensin family, is a small cationic peptide encoded by the DEFB106A gene located on human chromosome Yq11.223. Defensins are evolutionarily conserved antimicrobial peptides integral to innate immunity, functioning as first-line defenders against pathogens by disrupting microbial membranes and modulating immune responses. DEFB106A is primarily expressed in epithelial tissues, particularly in male reproductive organs like the testis, epididymis, and prostate, suggesting a role in reproductive tract immunity and spermatogenesis. Its expression is also detected in mucosal surfaces, such as the respiratory and urogenital tracts, highlighting broad-spectrum antimicrobial activity.
Recombinant DEFB106A protein is engineered through heterologous expression systems (e.g., E. coli or mammalian cells) to produce functional peptides for research and therapeutic applications. This involves cloning the DEFB106A gene into expression vectors, followed by purification using chromatography techniques like affinity tags (e.g., His-tag). The recombinant protein retains native bioactivity, including antibacterial, antifungal, and immunomodulatory properties. Studies suggest it interacts with chemokine receptors (e.g., CCR6) to recruit immune cells, bridging innate and adaptive immunity.
Research on DEFB106A focuses on its dual role in host defense and reproductive health. Dysregulation is linked to chronic inflammation, infections, and male infertility. Its recombinant form is explored as a potential therapeutic agent against multidrug-resistant pathogens or as a diagnostic biomarker. However, challenges remain in optimizing stability, delivery, and minimizing cytotoxicity. Further studies are needed to elucidate its molecular mechanisms and clinical potential.
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