| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PDLP7 |
| Uniprot No | Q0WPN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-298aa |
| 氨基酸序列 | TSATDTFVFGGCSQQKFSPASAYESNLNSLLTSLVNSATYSSYNNFTIMGSSSSDTARGLFQCRGDLSMPDCATCVARAVSQVGPLCPFTCGGALQLAGCYIKYDNISFLGQEDKTVVLKKCGSSEGYNTDGISRRDAVLTELVNGGGYFRAGGSGDVQGMGQCVGDLTVSECQDCLGTAIGRLKNDCGTAVFGDMFLAKCYARYSTDGAQHYAKSHNYKTNYGGEKTFAIIIGLLAAVVLLIIFLLFLRGVCSRGGDFSILHSFTLI |
| 预测分子量 | 41.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDLP7(Plasmodesmata-Located Protein 7)重组蛋白的3篇参考文献示例及摘要概括：
---
1. **文献名称**: *"Plant-specific DUF26-containing proteins regulate plasmodesmal immunity through receptor-like kinases"*
**作者**: Wang Y. et al. (2021)
**摘要**: 本文研究了PDLP7在植物细胞间通道(胞间连丝)免疫调控中的作用，发现其通过与受体激酶互作调控病原体防御信号传递。研究通过重组PDLP7蛋白验证了其体外结合病原相关分子的能力，揭示了其在植物先天免疫中的关键功能。
---
2. **文献名称**: *"Structural and functional analysis of PDLP7 in Arabidopsis plasmodesmata permeability"*
**作者**: Lee S. et al. (2019)
**摘要**: 通过重组表达纯化PDLP7胞外域蛋白，结合X射线晶体学解析其三维结构，发现其具有独特的β-折叠构象。功能实验表明，PDLP7通过调控胞间连丝通透性影响病毒颗粒的细胞间移动，为抗病毒育种提供新靶点。
---
3. **文献名称**: *"Recombinant PDLP7 fusion protein enhances plant resistance against bacterial pathogens"*
**作者**: Zhang R. et al. (2022)
**摘要**: 研究构建了PDLP7与几丁质酶融合的重组蛋白，在原核系统中高效表达并验证其抑菌活性。该蛋白可显著增强植物对假单胞菌的防御能力，为开发新型植物保护剂提供了实验依据。
---
注：以上文献信息为示例性内容，实际文献需通过数据库(如PubMed、Web of Science)以“PDLP7”或“Plasmodesmata-Located Protein 7”为关键词检索获取。如需真实文献，建议补充具体研究背景或应用场景。
PDLP7 (PLASMODESMATA-LOCALIZED PROTEIN 7) is a member of the plasmodesmata-localized protein family in plants, primarily associated with intercellular communication through plasmodesmata—microchannels connecting plant cells. These proteins play critical roles in regulating symplastic transport, including the movement of nutrients, signaling molecules, and pathogens. PDLP7 has garnered attention for its dual function in plant immunity and development. Studies suggest it acts as a susceptibility factor during pathogen infections, particularly in response to bacterial and fungal pathogens, by modulating plasmodesmal permeability or interacting with pathogen-derived effectors.
Recombinant PDLP7 protein is typically produced using heterologous expression systems (e.g., *E. coli* or yeast) to study its structural and functional properties. Its recombinant form enables biochemical assays, such as ligand-binding studies, and structural analyses (e.g., crystallography) to elucidate its molecular interactions. Structurally, PDLP7 contains conserved domains like the DUF26 motif and transmembrane regions, which are critical for its localization and role in membrane dynamics.
Research on recombinant PDLP7 has advanced understanding of plant-microbe interactions, revealing mechanisms by which pathogens manipulate host cells. For instance, PDLP7 overexpression has been linked to enhanced plasmodesmal closure, restricting viral spread, while its suppression may facilitate pathogen entry. This duality highlights its potential as a target for engineering disease-resistant crops. Additionally, recombinant PDLP7 serves as a tool to explore plasmodesmata regulation, offering insights into broader biological processes like developmental signaling and stress responses. Ongoing studies aim to dissect its interaction networks and exploit its biotechnological applications in agriculture.
×
