| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAST4 |
| Uniprot No | O15021 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1140-1231aa |
| 氨基酸序列 | QPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENT |
| 预测分子量 | 17.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAST4重组蛋白的3篇参考文献示例(注：部分文献信息为模拟，建议通过学术数据库核实具体内容)：
1. **"Expression and Purification of Recombinant MAST4 Kinase Domain for Structural Studies"**
*作者：Li, X. et al. (2018)*
**摘要**：报道了MAST4激酶结构域的重组表达与纯化方法，利用大肠杆菌系统优化表达条件，并通过X射线晶体学分析其三维结构，揭示了ATP结合位点特征。
2. **"Functional Characterization of MAST4 Recombinant Protein in Neuronal Signaling Pathways"**
*作者：Park, J. & Kim, S. (2020)*
**摘要**：研究重组MAST4蛋白在神经元中的功能，证实其通过磷酸化下游靶蛋白(如PTEN)调控AKT/mTOR通路，影响突触可塑性和细胞存活。
3. **"MAST4 Recombinant Kinase Activity Assay and Its Role in Breast Cancer Progression"**
*作者：Gupta, R. et al. (2021)*
**摘要**：开发了MAST4重组蛋白的体外激酶活性检测体系，并发现其过表达促进乳腺癌细胞侵袭，提示MAST4可能作为潜在治疗靶点。
**注意**：以上文献为示例，实际研究中建议通过PubMed或Web of Science检索最新论文。若需具体文献，可提供更详细的研究方向(如疾病模型、互作蛋白等)进一步筛选。
MAST4 (Microtubule-Associated Serine/Threonine Kinase 4) is a member of the MAST kinase family, characterized by a unique N-terminal domain of unknown function, a serine/threonine kinase domain, and a C-terminal PDZ domain that mediates protein-protein interactions. It plays roles in cellular processes such as cytoskeletal organization, signal transduction, and neuronal development. Dysregulation of MAST4 has been implicated in neurological disorders, cancers, and metabolic diseases, though its precise mechanisms remain under investigation.
Recombinant MAST4 protein is engineered using expression systems like *E. coli* or mammalian cell lines (e.g., HEK293) to ensure proper folding and post-translational modifications. The protein is often purified via affinity tags (e.g., His-tag) and validated through kinase activity assays and Western blotting. Researchers utilize recombinant MAST4 to study its enzymatic activity, substrate specificity, and interactions with signaling partners (e.g., MAPK pathways). It also serves as a tool for screening kinase inhibitors in drug discovery, particularly for targeting MAST4-overexpressing cancers.
Recent studies highlight MAST4’s role in synaptic plasticity and its potential as a biomarker in glioblastoma and Alzheimer’s disease models. Challenges include elucidating its regulatory networks and resolving structural details of its unique domains. Recombinant MAST4 remains critical for advancing both basic and translational research in cellular signaling and disease pathology.
×
