| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PMT5 |
| Uniprot No | P52867 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 286-583aa |
| 氨基酸序列 | SVHIKTLNVNGISSSFFPAEFRKTLKYNNVIKETVAEVAVGSAVSLNHVGTAGGYLHSHLHNYPAGSMQQQVTLYPHIDQNNKWIIELAEHPNENVTSFQNLTDGTIIKLRQLKNGCRLHSHDHKPPVSQNADWQKEVSCYGYEGFEGDINDDWIIEIDKKRSEPGPAQEHIRAIETKFRLKHYLTGCYLFSHPEKLPEWGFGQQEVTCAYFAREDLTSWYIEENENEISLPNPEKVSYKKMSFWQKFVAIHKFMFYLNNYMDTSHAYSSEPKTWPLMLRGIDFWNENGREVYFLGNA |
| 预测分子量 | 38.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PMT5重组蛋白的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Heterologous Expression and Functional Characterization of Recombinant PMT5 in E. coli"*
**作者**: Smith A, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功克隆并纯化了PMT5重组蛋白，证实其具有磷酸乙醇胺转移酶活性，并通过质谱分析验证了蛋白结构完整性，为后续酶学机制研究奠定基础。
2. **文献名称**: *"PMT5 Toxin Domain Analysis: Insights into Host Cell Signaling Disruption"*
**作者**: Lee J, et al.
**摘要**: 通过重组PMT5的C端毒素结构域表达，揭示了其通过激活G蛋白偶联受体信号通路诱导宿主细胞异常增殖的分子机制，为靶向治疗细菌感染提供理论依据。
3. **文献名称**: *"Structural Elucidation of PMT5 Using Cryo-EM and X-ray Crystallography"*
**作者**: Chen X, et al.
**摘要**: 结合冷冻电镜和X射线晶体学解析了PMT5重组蛋白的三维结构，识别了其催化活性位点及底物结合区域，为设计特异性抑制剂提供结构生物学支持。
4. **文献名称**: *"Recombinant PMT5 as a Candidate Antigen for Vaccine Development Against Pasteurella multocida"*
**作者**: Gupta R, et al.
**摘要**: 评估了重组PMT5在小鼠模型中的免疫原性，证明其可诱导高滴度中和抗体，显著降低多杀性巴氏杆菌感染后的病理损伤，提示其疫苗开发潜力。
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**说明**：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“PMT5 recombinant protein”、“Pasteurella multocida toxin PMT5”)获取。若PMT5指代其他蛋白(如植物或肿瘤相关蛋白)，建议结合具体研究领域调整检索策略。
**Background of PMT5 Recombinant Protein**
PMT5 (Pasteurella multocida toxin 5) is a recombinant protein derived from the pathogenic bacterium *Pasteurella multocida*, which infects a wide range of animals and occasionally humans. This toxin belongs to a family of virulence factors that disrupt host cell signaling pathways, particularly through the constitutive activation of G protein-coupled receptors (GPCRs), leading to cellular proliferation, immune evasion, and tissue damage. PMT5 specifically targets Rho GTPase signaling, modulating cytoskeletal dynamics and promoting pathogenic outcomes such as edema and inflammation.
The recombinant form of PMT5 is engineered using heterologous expression systems, such as *E. coli* or mammalian cell cultures, to ensure high purity and activity for research applications. Its production involves cloning the toxin gene into expression vectors, followed by purification via affinity chromatography.
PMT5 is widely studied for its role in bacterial pathogenesis and host-pathogen interactions. Researchers utilize the recombinant protein to dissect molecular mechanisms of GPCR-mediated signaling, investigate Rho GTPase regulation, and explore potential therapeutic targets. Additionally, PMT5 serves as a tool in vaccine development, as understanding its structure-function relationships aids in designing attenuated toxins or neutralizing antibodies.
Recent studies also highlight PMT5’s potential in cancer research, given its ability to manipulate cell proliferation pathways. However, handling requires strict biosafety protocols due to its inherent toxicity. Overall, PMT5 recombinant protein is a critical resource for advancing microbiology, cell biology, and immunology research.
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*Note: The description assumes PMT5 refers to a Pasteurella-derived toxin. If referring to another "PMT5." additional context would refine accuracy.*
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