| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KNSTRN |
| Uniprot No | Q9Y448 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-316aa |
| 氨基酸序列 | MAAPEAPPLDRVFRTTWLSTECDSHPLPPSYRKFLFETQAADLAGGTTVAAGNLLNESEKDCGQDRRAPGVQPCRLVTMTSVVKTVYSLQPPSALSGGQPADTQTRATSKSLLPVRSKEVDVSKQLHSGGPENDVTKITKLRRENGQMKATDTATRRNVRKGYKPLSKQKSEEELKDKNQLLEAVNKQLHQKLTETQGELKDLTQKVELLEKFRDNCLAILESKGLDPALGSETLASRQESTTDHMDSMLLLETLQEELKLFNETAKKQMEELQALKVKLEMKEERVRFLEQQTLCNNQVNDLTTALKEMEQLLEM |
| 预测分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KNSTRN重组蛋白的参考文献示例(注：部分内容为模拟示例，仅供参考)：
1. **文献名称**：*KNSTRN重组蛋白的克隆表达及其在细胞周期调控中的作用*
**作者**：Zhang Y, et al.
**摘要**：本研究成功构建了KNSTRN重组蛋白的原核表达系统，并通过体外实验证实其能够与微管蛋白结合，调控有丝分裂中染色体的正确分离，为探究KNSTRN在肿瘤发生中的机制提供了工具。
2. **文献名称**：*KNSTRN重组蛋白与SPAG5相互作用的功能分析*
**作者**：Lee S, Kim D.
**摘要**：通过重组KNSTRN蛋白的GST pull-down实验，发现其与SPAG5存在直接结合，并揭示两者复合物在维持纺锤体稳定性中的关键作用，为靶向细胞周期异常疾病的治疗提供新靶点。
3. **文献名称**：*乳腺癌中KNSTRN过表达的重组蛋白功能验证*
**作者**：Smith J, et al.
**摘要**：利用哺乳动物细胞表达系统制备KNSTRN重组蛋白，发现其过表达可促进乳腺癌细胞增殖，并通过干扰有丝分裂检查点导致基因组不稳定性，提示其作为潜在生物标志物的可能性。
4. **文献名称**：*KNSTRN蛋白晶体结构解析及其药物筛选平台构建*
**作者**：Wang X, et al.
**摘要**：通过重组KNSTRN蛋白的高效纯化与结晶，首次解析其三维结构，并基于此建立高通量抑制剂筛选模型，为开发抗有丝分裂类药物奠定基础。
**注**：以上文献为示例性内容，实际研究中需以具体数据库检索结果为准。
**Background of KNSTRN Recombinant Protein**
KNSTRN (kinetochore-localized astrin/SPAG5 binding protein), also known as C15orf23. is a key player in mitotic regulation, particularly during chromosome segregation. It localizes to kinetochores, dynamic protein structures that mediate interactions between chromosomes and spindle microtubules. KNSTRN interacts with astrin (SPAG5) to form the astrin-SKAP complex, which stabilizes kinetochore-microtubule attachments and ensures proper chromosome alignment during metaphase. This complex is essential for maintaining genomic stability by coordinating the spindle assembly checkpoint (SAC), a surveillance mechanism that delays anaphase until all chromosomes are correctly attached to the spindle.
KNSTRN’s role extends to regulating sister chromatid separation during anaphase. Its dysfunction is linked to chromosomal instability, a hallmark of cancer and developmental disorders. Studies highlight its overexpression in certain cancers, including breast and hepatocellular carcinomas, suggesting its potential as a therapeutic target or biomarker.
Recombinant KNSTRN protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and functional mechanisms. Produced via bacterial or mammalian expression systems, it retains post-translational modifications critical for its activity when expressed in eukaryotic hosts. Purification typically involves affinity tags (e.g., His-tag) for high yield and specificity.
Research applications include elucidating KNSTRN’s role in mitosis, screening inhibitors for cancer therapy, and studying its interplay with SAC components like MAD2 and BUBR1. Its recombinant form also aids in structural studies (e.g., X-ray crystallography) to map binding domains and validate interactions with astrin or microtubule-associated proteins.
Overall, KNSTRN recombinant protein serves as a vital tool for advancing our understanding of cell division mechanics and developing strategies to combat diseases driven by mitotic errors.
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