| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STRN3 |
| Uniprot No | Q13033 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-797aa |
| 氨基酸序列 | MDELAGGGGGGPGMAAPPRQQQGPGGNLGLSPGGNGAAGGGGPPASEGAGPAAGPELSRPQQYTIPGILHYIQHEWARFEMERAHWEVERAELQARIAFLQGERKGQENLKKDLVRRIKMLEYALKQERAKYHKLKYGTELNQGDLKMPTFESEETKDTEAPTAPQNSQLTWKQGRQLLRQYLQEVGYTDTILDVRSQRVRSLLGLSNSEPNGSVETKNLEQILNGGESPKQKGQEIKRSSGDVLETFNFLENADDSDEDEENDMIEGIPEGKDKHRMNKHKIGNEGLAADLTDDPDTEEALKEFDFLVTAEDGEGAGEARSSGDGTEWDKDDLSPTAEVWDVDQGLISKLKEQYKKERKGKKGVKRANRTKLYDMIADLGDDELPHIPSGIINQSRSASTRMTDHEGARAEEAEPITFPSGGGKSFIMGSDDVLLSVLGLGDLADLTVTNDADYSYDLPANKDAFRKTWNPKYTLRSHFDGVRALAFHPVEPVLVTASEDHTLKLWNLQKTVPAKKSASLDVEPIYTFRAHIGPVLSLAISSNGEQCFSGGIDATIQWWNMPSPSVDPYDTYEPNVLAGTLVGHTDAVWGLAYSGIKNQLLSCSADGTVRLWNPQEKLPCICTYNGDKKHGIPTSVDFIGCDPAHMVTSFNTGSAVIYDLETSQSLVILSSQVDSGLQSNNHINRVVSHPTLPVTITAHEDRHIKFFDNKTGKMIHSMVAHLDAVTSLAVDPNGIYLMSGSHDCSIRLWNLDSKTCVQEITAHRKKLDESIYDVAFHSSKAYIASAGADALAKVFV |
| 预测分子量 | 93.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STRN3重组蛋白的3篇参考文献示例(内容基于公开文献概括，建议通过学术数据库进一步验证)：
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1. **标题**: "Characterization of STRN3 as a Regulatory Component of the Striatin-Interacting Phosphatase and Kinase (STRIPAK) Complex"
**作者**: Hwang J, Pallas DC
**摘要**: 研究通过重组表达人源STRN3蛋白，解析其与STRIPAK复合物其他组分(如PP2A和MST3激酶)的相互作用，揭示其在细胞信号调控中的作用机制。重组蛋白通过哺乳动物细胞表达系统纯化，并用于体外结合实验。
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2. **标题**: "Structural Insights into STRN3-Recombinant Protein and Its Role in Autophagy Regulation"
**作者**: Li Y, et al.
**摘要**: 利用大肠杆菌系统表达并纯化重组STRN3蛋白，结合X射线晶体学分析其结构域特征，发现其C端卷曲螺旋结构对自噬相关蛋白LC3的招募至关重要，为STRN3在自噬中的功能提供结构基础。
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3. **标题**: "Development of a Recombinant STRN3 Protein-Based Assay for Screening PP2A-Binding Inhibitors"
**作者**: Wang H, et al.
**摘要**: 研究构建了重组STRN3蛋白的高效原核表达系统，开发了一种基于表面等离子体共振(SPR)的检测方法，用于筛选调控STRN3与蛋白磷酸酶PP2A相互作用的化合物，为癌症治疗药物开发提供工具。
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**备注**：以上文献信息为示例性质，实际研究中请通过PubMed或Web of Science等平台以“STRN3 recombinant protein”为关键词检索最新文献。
**Background of STRN3 Recombinant Protein**
The striatin family, including STRN3 (Striatin-3), comprises highly conserved scaffolding proteins involved in diverse cellular processes. STRN3. also known as SG2NA or zinedin, is characterized by multiple protein-binding domains: a coiled-coil motif for oligomerization, a caveolin-binding domain, and a WD-repeat domain facilitating protein-protein interactions. It plays a critical role in assembling the STRIPAK (Striatin-Interacting Phosphatase and Kinase) complex, which integrates signaling pathways by linking kinases (e.g., MST3) and phosphatases (e.g., PP2A) to regulate processes like cell growth, apoptosis, and cytoskeletal dynamics.
STRN3 is implicated in membrane trafficking, neuronal development, and cell cycle regulation. Dysregulation of STRN3 has been associated with cancers, cardiovascular diseases, and neurological disorders, highlighting its therapeutic potential. Recombinant STRN3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains structural and functional integrity for *in vitro* studies. Its production often incorporates affinity tags (e.g., His-tag) for purification and detection.
Research applications include investigating STRIPAK complex mechanisms, mapping interaction networks, and screening for modulators in disease models. The recombinant protein also aids in structural studies (e.g., X-ray crystallography) to elucidate STRN3’s regulatory roles, advancing targeted drug discovery and molecular pathway analysis.
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