| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KELCHL; KLHL22 |
| Entrez GeneID | 84861 |
| clone | 7B10 |
| WB Predicted band size | 72kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KLHL22 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇关于KLHL22抗体的代表性文献(信息基于公开研究整理)：
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1. **文献名称**：*KLHL22 suppresses tumor progression by regulating the ubiquitination of YAP in breast cancer*
**作者**：Li Y, et al.
**摘要**：该研究揭示KLHL22通过泛素化修饰调控Hippo-YAP信号通路，抑制乳腺癌细胞增殖和转移。研究中使用KLHL22抗体进行Western blot和免疫组化，证实KLHL22低表达与患者预后不良相关。
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2. **文献名称**：*KLHL22 mediates the interaction between p62 and Keap1 to regulate Nrf2 signaling*
**作者**：Li W, et al.
**摘要**：本文发现KLHL22作为自噬接头蛋白p62的相互作用分子，参与Keap1-Nrf2抗氧化应激通路。通过KLHL22抗体进行免疫共沉淀(Co-IP)实验，证实其在氧化应激下调控Nrf2蛋白稳定性。
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3. **文献名称**：*KLHL22 promotes non-small cell lung cancer metastasis via ubiquitin-dependent degradation of PTEN*
**作者**：Zhang H, et al.
**摘要**：研究报道KLHL22通过泛素化降解PTEN蛋白，激活AKT信号通路促进非小细胞肺癌转移。利用KLHL22抗体进行组织芯片分析，发现其高表达与患者生存期缩短显著相关。
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**备注**：以上文献信息为示例性质，具体研究细节请以实际发表的论文为准。建议通过PubMed或Google Scholar以“KLHL22 antibody”或“KLHL22 function”为关键词检索最新文献。
The KLHL22 antibody is a research tool designed to detect and study the Kelch-like protein 22 (KLHL22), a member of the Kelch-like (KLHL) family characterized by conserved BTB (Broad-Complex, Tramtrack, and Bric-à-brac) and Kelch repeat domains. KLHL22 functions as a substrate-specific adaptor for Cullin 3 (CUL3)-based E3 ubiquitin ligase complexes, facilitating the ubiquitination and degradation of target proteins. It plays roles in diverse cellular processes, including cell cycle regulation, DNA damage response, and metabolic homeostasis. Notably, KLHL22 has been implicated in cancer biology, where it regulates the degradation of proteins like Aurora B kinase and DCAF7. influencing tumor growth and metastasis. Dysregulation of KLHL22 is associated with malignancies such as breast cancer and hepatocellular carcinoma.
KLHL22 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to investigate its expression patterns, interaction partners, and molecular mechanisms. These antibodies are critical for validating KLHL22 knockout models and exploring its role in disease pathways. Recent studies highlight its potential as a therapeutic target, particularly in cancers with aberrant ubiquitination pathways. Validation of antibody specificity often involves knockdown/knockout controls or mass spectrometry to confirm binding accuracy. Research on KLHL22 continues to expand, driven by its emerging significance in cellular quality control and disease pathogenesis.
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