| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GGPS1 |
| Uniprot No | O95749 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-300aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEKTQETVQRILLEPYKYLLQLPGKQVRTK LSQAFNHWLKVPEDKLQIIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAH SIYGIPSVINSANYVYFLGLEKVLTLDHPDAVKLFTRQLLELHQGQGLDI YWRDNYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLNTL GLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQV QNILRQRTENIDIKKYCVHYLEDVGSFEYTRNTLKELEAKAYKQIDARGG NPELVALVKHLSKMFKEENE |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **GGPS1(香叶基香叶基焦磷酸合成酶1)重组蛋白** 的参考文献示例，包含文献名称、作者及摘要内容概括：
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1. **《Geranylgeranyl pyrophosphate synthase 1 modulates tumor progression via RhoA regulation in hepatocellular carcinoma》**
*作者：Zhang Y, et al. (2020)*
**摘要**：本研究通过表达重组GGPS1蛋白，发现其通过调控RhoA蛋白的异戊二烯化修饰，促进肝癌细胞迁移和侵袭，揭示GGPS1在肿瘤转移中的关键作用。
2. **《Functional characterization of recombinant human GGPS1 in cholesterol metabolism》**
*作者：Li X, et al. (2018)*
**摘要**：利用大肠杆菌系统成功表达并纯化重组人源GGPS1蛋白，体外酶活实验表明其催化香叶基焦磷酸(GPP)转化为GGPP的效率受氧化应激调控，提示其在胆固醇合成中的动态调节机制。
3. **《Crystal structure and enzymatic analysis of GGPS1 from Arabidopsis thaliana》**
*作者：Kim S, et al. (2016)*
**摘要**：解析拟南芥GGPS1重组蛋白的晶体结构，结合突变实验发现其活性位点关键氨基酸残基(如Asp95和Lys155)，为靶向GGPS1的植物代谢工程提供结构基础。
4. **《GGPS1-mediated protein geranylgeranylation exacerbates tau pathology in Alzheimer's disease models》**
*作者：Wang L, et al. (2022)*
**摘要**：通过重组GGPS1蛋白处理神经元细胞，发现其增强tau蛋白的异戊二烯化修饰，导致神经纤维缠结加剧，提示GGPS1抑制剂可能作为阿尔茨海默病的潜在治疗策略。
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以上文献方向涵盖 **癌症、代谢疾病、结构生物学及神经退行性疾病**，具体研究内容可根据实际需求调整。如需真实文献，建议通过PubMed或Web of Science检索关键词“GGPS1 recombinant protein”。
**Background of GGPS1 Recombinant Protein**
Geranylgeranyl diphosphate synthase 1 (GGPS1) is a key enzyme in the mevalonate pathway, responsible for catalyzing the synthesis of geranylgeranyl pyrophosphate (GGPP), a critical isoprenoid metabolite. GGPP serves as a lipid donor for protein geranylgeranylation, a post-translational modification essential for membrane localization and function of small GTPases, such as Rho and Rab family proteins, which regulate diverse cellular processes including cytoskeletal organization, vesicle trafficking, and cell signaling.
GGPS1 is a member of the prenyltransferase family and operates by condensing isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to produce GGPP. Its enzymatic activity is tightly regulated, linking it to metabolic homeostasis and diseases like cancer, cardiovascular disorders, and osteoporosis. Dysregulation of GGPP levels due to altered GGPS1 expression can disrupt protein prenylation, contributing to pathological cell proliferation, migration, or apoptosis.
Recombinant GGPS1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable high-purity, functional enzyme production for research. It is widely used to study enzymatic mechanisms, screen inhibitors, or explore therapeutic strategies targeting protein prenylation. Additionally, recombinant GGPS1 aids in elucidating its structural and functional roles in metabolic diseases and age-related conditions, where GGPP-dependent pathways are implicated.
As a tool in biotechnology, recombinant GGPS1 also supports the biosynthesis of isoprenoid-derived compounds, highlighting its dual significance in basic research and applied sciences.
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