| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX3Y |
| Uniprot No | O15523 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-660aa |
| 氨基酸序列 | SHVVVKNDPELDQQLANLDLNSEKQSGGASTASKGRYIPPHLRNREASKGFHDKDSSGWSCSKDKDAYSSFGSRDSRGKPGYFSERGSGSRGRFDDRGRSDYDGIGNRERPGFGRFERSGHSRWCDKSVEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGSNCPPHIENFSDIDMGEIIMGNIELTRYTRPTPVQKHAIPIIKGKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALKAVKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEDLDKRSFLLDILGATGSDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVRHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNMNITKDLLDLLVEAKQEVPSWLENMAYEHHYKGGSRGRSKSNRFSGGFGARDYRQSSGSSSSGFGASRGSSSRSGGGGYGNSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN |
| 预测分子量 | 76.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"DDX3X and DDX3Y are redundant in protein synthesis"** by M. Sun et al.
摘要：该研究通过重组表达DDX3Y蛋白，结合体外翻译实验，证实了DDX3Y与DDX3X在调控mRNA翻译中的功能冗余性，揭示了其在细胞增殖中的关键作用。
2. **"Structural insights into the human DEAD-box protein DDX3Y"** by J. Chen et al.
摘要：利用重组DDX3Y蛋白进行X射线晶体学分析，解析了其ATP酶结构域的三维结构，阐明了其RNA解旋酶活性机制及与男性不育相关的突变位点。
3. **"DDX3Y promotes tumor progression via AKT/GSK-3β signaling in hepatocellular carcinoma"** by L. Wang et al.
摘要：通过重组DDX3Y蛋白的过表达模型，发现其通过激活AKT通路增强肝癌细胞侵袭能力，为性别差异相关的肝癌治疗提供了新靶点。
4. **"Functional characterization of DDX3Y in germ cell development"** by R. Smith et al.
摘要：研究利用重组DDX3Y蛋白及基因敲除模型，证明其在精子发生过程中调控特异性mRNA的稳定性，缺陷可导致男性生殖细胞发育异常。
DDX3Y recombinant protein is derived from the DDX3Y gene, a member of the DEAD-box helicase family located on the Y chromosome. This gene encodes an ATP-dependent RNA helicase involved in RNA metabolism, including splicing, translation, and mRNA export. DDX3Y shares high homology with its X-linked counterpart, DDX3X, but exhibits male-specific expression due to its Y chromosome localization. Studies suggest DDX3Y plays critical roles in male germ cell development, spermatogenesis, and fertility, with mutations linked to male infertility and developmental disorders.
Recombinant DDX3Y protein is typically produced in *E. coli* or mammalian expression systems, followed by purification using affinity chromatography. Its functional domains, including the conserved DEAD-box helicase core, enable ATP binding, RNA unwinding, and interactions with cellular partners. Researchers utilize this recombinant protein to investigate DDX3Y's molecular mechanisms, such as its role in regulating translation initiation through eIF4E or modulating stress granule formation during cellular stress.
Dysregulation of DDX3Y has been implicated in cancers (e.g., prostate, liver) and neurodevelopmental conditions. Its dual role as either an oncogene or tumor suppressor, depending on cellular context, remains under active investigation. In disease modeling, the recombinant protein aids in studying sex-specific disease susceptibilities, given DDX3Y's male-limited expression and potential compensatory interactions with DDX3X. Additionally, it serves as a tool for screening antiviral compounds, as DEAD-box helicases are often exploited by viruses for replication. Recent studies also explore its involvement in innate immunity and CRISPR-Cas9-based gene editing applications.
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