| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FTO |
| Uniprot No | Q9C0B1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-505aa |
| 氨基酸序列 | TPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRD LVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAE IAAACETFLKLNDYLQIETIQALEELAAKEKANEDAVPLCMSADFPRVGM GSSYNGQDEVDIKSRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWH HDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDIWHVGFKISWDIE TPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTGTL DYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQ FWFQGNRYRKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQR NEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDA SMPLPFDLTDIVSELRGQLLEAKP |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FTO重组蛋白的3篇代表性文献，涵盖其结构、功能及疾病关联研究：
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1. **文献名称**：*N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO*
**作者**：Jia, G., et al.
**摘要**：该研究首次揭示了FTO蛋白通过催化RNA中N6-甲基腺苷(m6A)的去甲基化作用调控基因表达，并解析了重组FTO蛋白的酶活机制，阐明了其与肥胖相关的分子基础。
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2. **文献名称**：*Crystal structure of the FTO protein reveals basis for its substrate specificity*
**作者**：Han, Z., et al.
**摘要**：通过X射线晶体学解析了重组人源FTO蛋白的三维结构，阐明了其底物识别及催化机制，为开发靶向FTO的小分子抑制剂提供了结构基础。
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3. **文献名称**：*FTO regulates adipogenesis by controlling cell cycle progression via m6A demethylation*
**作者**：Zheng, G., et al.
**摘要**：利用重组FTO蛋白进行体外实验，证明其通过去除脂肪细胞分化相关基因mRNA的m6A修饰，调控细胞周期进程，进而影响肥胖相关代谢通路。
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这些研究共同揭示了FTO重组蛋白在表观遗传调控及代谢疾病中的关键作用。
**Background of FTO Recombinant Protein**
The fat mass and obesity-associated (FTO) protein, encoded by the *FTO* gene, is an enzyme belonging to the AlkB family of non-heme Fe(II)/α-ketoglutarate-dependent dioxygenases. Initially linked to obesity and metabolic disorders through genome-wide association studies, FTO gained attention for its role in regulating energy homeostasis and adipogenesis. Subsequent research revealed its enzymatic activity as an RNA demethylase, primarily targeting N6-methyladenosine (m6A) and other methyl modifications in RNA, influencing mRNA splicing, stability, and translation. This epigenetic regulatory function positions FTO as a critical player in cellular processes, including cell differentiation, proliferation, and stress responses.
Recombinant FTO protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, enzymatic mechanisms, and interactions. Purification typically involves affinity tags (e.g., His-tag) and chromatographic techniques. Its recombinant form enables *in vitro* assays to screen inhibitors or modulators, aiding drug development for obesity-related diseases, cancers, and neurological disorders linked to FTO dysregulation.
Current research focuses on resolving FTO's substrate specificity, structural dynamics, and tissue-specific roles. Challenges include understanding its dual cytoplasmic/nuclear localization and context-dependent effects on gene expression. Recombinant FTO tools are pivotal in dissecting its pathophysiological roles and advancing targeted therapies, particularly for diseases driven by aberrant RNA methylation.
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