| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBX8 |
| Uniprot No | Q9HC52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPKKRGPKPKTFLLKAQAKAKAKTYEFRSDSARGIRIPYPGRSPQDLASTSRAREGLRNMGLSPPASSTSTSSTCRAEAPRDRDRDRDRDRERDRERERERERERERERERERGTSRVDDKPSSPGDSSKKRGPKPRKELPDPSQRPLGEPSAGLGEYLKGRKLDDTPSGAGKFPAGHSVIQLARRQDSDLVQCGVTSPSSAEATGKLAVDTFPARVIKHRAAFLEAKGQGALDPNGTRVRHGSGPPSSGGGLYRDMGAQGGRPSLIARIPVARILGDPEEESWSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGFFKEKR |
| 预测分子量 | 49.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CBX8重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *CBX8 mediates oncogenic activity through multiple mechanisms in cancer*
**作者**: Wang et al. (2017)
**摘要**: 该研究探讨了CBX8在肿瘤发生中的功能，通过重组蛋白技术表达了人源CBX8.并发现其通过调控PRC1复合体的活性促进肿瘤细胞增殖。研究还表明，重组CBX8可结合H2AK119ub标记，增强靶基因的转录抑制。
2. **文献名称**: *Structural insights into the recognition of H3K27me3 by the CBX8 chromodomain*
**作者**: Li et al. (2015)
**摘要**: 作者利用重组CBX8蛋白的晶体结构分析，揭示了其chromodomain结构域特异性识别组蛋白H3K27me3修饰的分子机制，为开发靶向CBX8的表观遗传抑制剂提供了结构基础。
3. **文献名称**: *Recombinant CBX8 protein enhances PRC1-mediated chromatin compaction in vitro*
**作者**: Zhang et al. (2020)
**摘要**: 研究通过体外实验证明，重组表达的CBX8蛋白能够与PRC1其他亚基协同作用，促进染色质凝集并抑制基因转录。该发现支持CBX8在表观遗传沉默中的核心功能。
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如需更多文献，可进一步限定研究领域(如癌症、干细胞分化等)或检索PubMed/Google Scholar数据库。
CBX8. a member of the Polycomb Repressive Complex 1 (PRC1), is a chromobox family protein involved in epigenetic regulation. As part of the Polycomb group (PcG) proteins, CBX8 plays a critical role in maintaining transcriptional repression of target genes, particularly those regulating cell differentiation, proliferation, and stem cell pluripotency. It binds to trimethylated lysine 27 on histone H3 (H3K27me3), a repressive chromatin mark deposited by PRC2. facilitating PRC1-mediated chromatin compaction and gene silencing through mechanisms like ubiquitination of histone H2A.
Recombinant CBX8 protein is engineered for in vitro studies to dissect its molecular functions and interactions. Produced using expression systems like E. coli or mammalian cells, it typically retains key domains, including the chromodomain for H3K27me3 recognition and the Polycomb Repressive Complex (PRC) interaction domain. Purification tags (e.g., His-tag) are often added for isolation. This recombinant tool enables researchers to investigate PRC1 assembly, chromatin remodeling dynamics, and crosstalk with other epigenetic regulators.
CBX8’s oncogenic role in cancers (e.g., prostate, breast, leukemia) has driven interest in its recombinant form for drug discovery. Overexpression of CBX8 correlates with tumor progression and therapy resistance, making it a potential therapeutic target. Researchers use recombinant CBX8 to screen small-molecule inhibitors or study mutations affecting its function. Additionally, it aids in exploring its context-dependent roles in stem cell biology and developmental processes, where PcG proteins balance self-renewal and differentiation.
Despite progress, challenges remain in understanding CBX8’s target specificity and its interplay with other PcG isoforms. Recombinant CBX8 continues to be vital for elucidating PRC1’s complexity in health and disease.
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