| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POTEKP |
| Uniprot No | Q9BYX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MDDDTAVLVIDNGSGMCKAGFAGDDAPQAVFPSIVGRPRHQGMMEGMHQKESYVGKEAQSKRGMLTLKYPMEHGIITNWDDMEKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYTSGRTTGIVMDSGDGFTHTVPIYEGNALPHATLRLDLAGRELTDYLMKILTERGYRFTTTAEQEIVRDIKEKLCYVALDSEQEMAMAASSSSVEKSYELPDGQVITIGNERFRCPEALFQPCFLGMESCGIHKTTFNSIVKSDVDIRKDLYTNTVLSGGTTMYPGIAHRMQKEITALAPSIMKIKIIAPPKRKYSVWVGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF |
| 预测分子量 | 49.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：POTEKP重组蛋白在肿瘤细胞凋亡调控中的作用研究
**作者**：Chen L, Wang Y
**摘要**：本研究探讨了POTEKP重组蛋白通过调节Bcl-2/Bax通路诱导前列腺癌细胞凋亡的机制，证实其可抑制肿瘤生长并增强化疗敏感性。
2. **文献名称**：重组蛋白POTEKP的晶体结构解析及功能验证
**作者**：Zhang R, et al.
**摘要**：通过X射线衍射解析POTEKP重组蛋白的三维结构，揭示其与细胞膜受体的结合域，为靶向药物设计提供结构基础。
3. **文献名称**：POTEKP重组蛋白在乳腺癌中的免疫调节功能
**作者**：Kim S, Patel D
**摘要**：实验表明POTEKP重组蛋白通过激活树突状细胞增强抗肿瘤免疫应答，显著抑制小鼠模型中乳腺癌转移。
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**备注**：以上文献为示例，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。若需具体文献，建议使用关键词“POTEKP recombinant protein”或“POTE gene family”进行检索。
POTEKP is a recombinant protein derived from the POTE gene family, a group of primate-specific genes implicated in various cellular processes and disease pathways. The POTE (PRAME, OB-fold, and TEP1) genes are characterized by their unique structural domains, including actin-binding motifs and ankyrin repeats, which suggest roles in cytoskeletal organization and protein-protein interactions. These genes are expressed in a tissue-specific manner, with elevated levels observed in cancers (e.g., breast, prostate, and ovarian tumors), making them potential biomarkers or therapeutic targets.
The POTEKP variant, generated through recombinant DNA technology, typically involves cloning the POTE coding sequence into expression vectors (e.g., bacterial or mammalian systems) to produce purified protein for functional studies. Its recombinant form allows researchers to investigate its biochemical properties, interaction partners, and signaling mechanisms in controlled settings. Studies indicate that POTE proteins may influence apoptosis, cell proliferation, and immune responses, though their exact physiological roles remain under exploration. POTEKP, in particular, has been leveraged to study its immunogenic potential, as POTE-derived antigens can elicit immune responses in cancer models, suggesting utility in vaccine development or immunotherapy.
Current research focuses on elucidating POTEKP's structural-functional relationships, its cross-talk with oncogenic pathways (e.g., PI3K/AKT or Wnt/β-catenin), and its diagnostic relevance in liquid biopsies. Challenges include understanding its tissue-specific regulation and resolving conflicting data on its pro- or anti-tumor effects. Despite these gaps, POTEKP exemplifies how recombinant proteins enable targeted inquiry into enigmatic gene families, bridging genomic discoveries to translational applications in oncology and molecular medicine.
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