| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JBP1 |
| Uniprot No | O14744 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-637aa |
| 氨基酸序列 | MAAMAVGGAG GSRVSSGRDL NCVPEIADTL GAVAKQGFDF LCMPVFHPRF KREFIQEPAK NRPGPQTRSD LLLSGRDWNT LIVGKLSPWI RPDSKVEKIR RNSEAAMLQE LNFGAYLGLP AFLLPLNQED NTNLARVLTN HIHTGHHSSM FWMRVPLVAP EDLRDDIIEN APTTHTEEYS GEEKTWMWWH NFRTLCDYSK RIAVALEIGA DLPSNHVIDR WLGEPIKAAI LPTSIFLTNK KGFPVLSKMH QRLIFRLLKL EVQFIITGTN HHSEKEFCSY LQYLEYLSQN RPPPNAYELF AKGYEDYLQS PLQPLMDNLE SQTYEVFEKD PIKYSQYQQA IYKCLLDRVP EEEKDTNVQV LMVLGAGRGP LVNASLRAAK QADRRIKLYA VEKNPNAVVT LENWQFEEWG SQVTVVSSDM REWVAPEKAD IIVSELLGSF ADNELSPECL DGAQHFLKDD GVSIPGEYTS FLAPISSSKL YNEVRACREK DRDPEAQFEM PYVVRLHNFH QLSAPQPCFT FSHPNRDPMI DNNRYCTLEF PVEVNTVLHG FAGYFETVLY QDITLSIRPE THSPGMFSWF PILFPIKQPI TVREGQTICV RFWRCSNSKK VWYEWAVTAP VCSAIHNPTG RSYTIGL |
| 预测分子量 | 72,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于JBP1重组蛋白的3篇模拟参考文献(基于领域知识，非真实文献)：
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1. **文献名称**: *Cloning and functional characterization of JBP1 in Trypanosoma brucei*
**作者**: Cross, S.H., et al.
**摘要**: 本研究通过重组表达锥虫JBP1蛋白，揭示了其在碱基J(β-D-葡萄糖基羟甲基尿嘧啶)合成中的关键作用。实验表明，重组JBP1具有特异性结合羟化酶的功能，并参与调控DNA修饰过程。
2. **文献名称**: *JBP1 and JBP2: Divergent roles in kinetoplastid DNA modification*
**作者**: Cliffe, L.J., et al.
**摘要**: 通过比较重组JBP1与JBP2蛋白的活性，发现JBP1主要作用于DNA羟化反应的早期步骤，而JBP2则参与后续糖基化修饰，表明二者在碱基J合成通路中的协同作用。
3. **文献名称**: *Structural analysis of JBP1 reveals a conserved SWIRM domain essential for DNA binding*
**作者**: Genest, P.A., et al.
**摘要**: 利用重组JBP1蛋白进行结构域解析，发现其SWIRM结构域对DNA结合至关重要。该研究通过点突变实验证实，SWIRM域的缺失会完全破坏JBP1的DNA修饰功能。
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**注意**：以上文献为模拟示例，实际引用时需核实真实文献来源。建议通过PubMed或Google Scholar检索关键词“JBP1 recombinant protein”或“JBP1 Trypanosoma”获取准确信息。
JBP1 (J-binding protein 1) is a DNA-binding protein initially identified in *Trypanosoma brucei*, a parasitic protozoan responsible for African trypanosomiasis. Its name derives from its ability to specifically recognize and bind to the unusual DNA base "J" (β-D-glucosyl-hydroxymethyluracil), a thymine derivative unique to kinetoplastid organisms. This hypermodified base is enriched in telomeric regions and transcriptionally silent gene loci, suggesting a role in epigenetic regulation and genome stability. JBP1 facilitates the biosynthesis and maintenance of base J by promoting the hydroxymethylation of thymine residues, a process critical for modulating chromatin structure and gene expression in trypanosomes.
Recombinant JBP1 protein is engineered for in vitro and in vivo studies to dissect its molecular functions. Typically produced in *E. coli* or eukaryotic expression systems, the recombinant protein retains conserved domains essential for activity, including the SWIM zinc finger motif for DNA interaction and a J-binding domain. Researchers utilize it to investigate J-base biosynthesis pathways, DNA-protein interactions, and epigenetic silencing mechanisms in trypanosomes. Its applications extend to drug discovery, as disrupting JBP1 function could impair parasite survival, offering a potential therapeutic strategy against trypanosomiasis. Additionally, recombinant JBP1 serves as a tool to explore conserved epigenetic processes in other organisms, given parallels between base J and hydroxymethylcytosine in mammalian systems. Studies also leverage it to develop molecular probes for detecting J-base modifications, advancing understanding of non-canonical DNA modifications in microbial pathogenesis and evolution.
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