首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGF1 |
| Uniprot No | Q3E880 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 104-116aa |
| 氨基酸序列 | DYSNPGHHPPRHN |
| 预测分子量 | 28.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGF1重组蛋白的3篇参考文献示例(注：部分信息为模拟内容，实际文献需根据具体研究补充)：
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1. **文献名称**：*Heterologous Expression and Functional Analysis of Recombinant RGF1 in Arabidopsis Root Development*
**作者**：K. Matsubayashi et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备了重组RGF1蛋白，并通过体外生物活性实验证实其能够特异性激活拟南芥根尖分生组织的细胞增殖，揭示了RGF1通过受体激酶RGFR1介导的信号通路调控根系发育的分子机制。
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2. **文献名称**：*Crystal Structure of RGF1 Peptide Bound to Its Receptor Reveals Signaling Specificity*
**作者**：X. Li et al.
**摘要**：通过昆虫细胞系统表达并纯化RGF1及其受体复合物，结合X射线晶体学解析了二者结合的三维结构，阐明了RGF1的C端保守序列对受体激活的关键作用，为设计植物生长调节剂提供了结构基础。
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3. **文献名称**：*Optimization of RGF1 Recombinant Production in Plant Cell Suspension Cultures for Agricultural Applications*
**作者**：S. Rodriguez et al.
**摘要**：开发了一种基于植物悬浮细胞的重组RGF1规模化生产方法，证明了该蛋白在玉米和大豆中显著提高根系生物量及抗旱能力，为作物改良提供了新策略。
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**注意**：以上文献信息为示例性质，实际引用请以真实发表的论文为准。建议通过PubMed、Web of Science或Google Scholar以关键词“RGF1 recombinant protein”、“RGF1 peptide signaling”等检索最新研究。
**Background of RGF1 Recombinant Protein**
RGF1 (Root Meristem Growth Factor 1), also known as GOLVEN1 or CLEL6. is a plant peptide hormone critical for root development. It was first identified in *Arabidopsis thaliana* as a member of the RGF/GLV/CLEL family, which comprises secreted cysteine-rich peptides involved in cell-to-cell communication. RGF1 specifically regulates root stem cell niche maintenance and root meristem activity, ensuring proper root elongation and architecture.
Biochemically, RGF1 is synthesized as a precursor protein that undergoes post-translational modifications, including sulfation and proteolytic processing, to generate the mature bioactive peptide. It binds to receptor-like kinases (RLKs) on target cells, such as the RGF1 INSENSITIVE (RGI) receptors, triggering downstream signaling cascades involving reactive oxygen species (ROS), mitogen-activated protein kinases (MAPKs), and the PLETHORA (PLT) transcription factors. These pathways collectively modulate stem cell differentiation and division rates in the root apical meristem.
The recombinant RGF1 protein is produced via heterologous expression systems (e.g., *E. coli* or yeast) to study its structure-function relationships, receptor interactions, and agricultural applications. Its role in enhancing root growth under stress conditions has sparked interest in biotechnology for improving crop nutrient uptake and drought resistance. Research on RGF1 also provides insights into conserved peptide signaling mechanisms across plant species, bridging developmental biology and sustainable agriculture.
In summary, RGF1 recombinant protein serves as a key tool to dissect root developmental processes and explore its biotechnological potential in enhancing plant resilience and productivity.
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