| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIP |
| Uniprot No | O00170 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHPVWETIVCTMREGEIAQFLCDIKHVVLYPL VAKSLRNIAVGKDPLEGQRHCCGVAQMREHSSLGHADLDALQQNPQPLIF HMEMLKVESPGTYQQDPWAMTDEEKAKAVPLIHQEGNRLYREGHVKEAAA KYYDAIACLKNLQMKEQPGSPEWIQLDKQITPLLLNYCQCKLVVEEYYEV MADIIARLREDGIQKRVIQEGRGELPDFQDGTKATFHYRTLHSDDEGTVL DDSRARGKPMELIIGKKFKLLDHCSSILNKYDDNVKAYFKRGKAHAAVWN AQEAQADFAKVLELDPALAPVVSRELRALEARIRQKDEEDKARFRGIFSH |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AIP(芳香烃受体相互作用蛋白)重组蛋白研究的模拟参考文献示例(仅供参考，具体文献请通过学术数据库查询)：
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1. **文献名称**：*Expression and Purification of Recombinant AIP in Escherichia coli for Functional Studies*
**作者**：Smith J, et al.
**摘要**：本研究成功构建了AIP的重组表达载体，利用大肠杆菌系统高效表达并纯化具有生物活性的AIP蛋白。通过Western blot和圆二色谱验证了蛋白的正确折叠，为后续研究AIP的相互作用及功能提供了可靠工具。
2. **文献名称**：*Structural Insights into AIP Mutations Linked to Pituitary Adenomas*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了AIP及其致病突变体的三维结构，揭示了突变导致AIP与芳香烃受体(AhR)结合能力下降的分子机制，为垂体瘤的病理机制研究提供了结构基础。
3. **文献名称**：*Functional Characterization of Recombinant AIP in cAMP Signaling Pathway*
**作者**：Wang Y, et al.
**摘要**：利用重组AIP蛋白探究其在细胞cAMP信号通路中的作用，发现AIP通过调控磷酸二酯酶活性影响激素分泌，提示其可能作为内分泌肿瘤治疗的潜在靶点。
4. **文献名称**：*Development of an AIP-Based Biosensor for Environmental Toxin Detection*
**作者**：Kim S, et al.
**摘要**：基于重组AIP蛋白与AhR的相互作用，构建了一种新型生物传感器，可高效检测环境中二噁英类污染物，为环境监测提供了高灵敏度工具。
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**提示**：实际文献需通过PubMed、Web of Science等平台检索关键词如“AIP recombinant protein”、“Aryl hydrocarbon receptor-interacting protein expression”获取，并优先选择近五年内发表的论文以反映最新进展。
Aryl hydrocarbon receptor-interacting protein (AIP), a 37 kDa immunophilin-like protein, plays a critical role in cellular signaling pathways and disease pathogenesis. Initially identified through its interaction with the aryl hydrocarbon receptor (AhR), AIP functions as a molecular chaperone, stabilizing client proteins like AhR and the glucocorticoid receptor. It is ubiquitously expressed but notably enriched in endocrine tissues, particularly the pituitary gland. Germline mutations in the AIP gene are linked to familial isolated pituitary adenomas (FIPA), predisposing individuals to growth hormone-secreting or prolactinomas, often with early-onset and aggressive phenotypes. The protein’s tumor-suppressive role involves regulating cyclic AMP signaling, protein stability, and mitochondrial apoptosis, though its precise mechanisms remain under investigation.
Recombinant AIP proteins, produced via bacterial (e.g., E. coli) or eukaryotic expression systems, are essential tools for structural and functional studies. These proteins retain key domains, including the N-terminal tetratricopeptide repeat (TPR) motif for protein interactions and the C-terminal α-helix. Purification methods typically employ affinity tags (e.g., His-tag) followed by chromatographic refinement. Recombinant AIP enables in vitro analyses of its interaction networks, post-translational modifications, and mutant variants associated with disease. It also serves in drug screening platforms targeting AIP-deficient tumors. Despite challenges in maintaining solubility and native conformation during production, advances in protein engineering and codon optimization continue to enhance recombinant AIP’s utility in both basic research and therapeutic development, particularly for pituitary disorders and AhR-related environmental toxin responses.
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