| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSN1S2 |
| Uniprot No | P02663 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-222aa |
| 氨基酸序列 | MKFFIFTCLLAVALAKNTMEHVSSSEESIISQETYKQEKNMAINPSKENLCSTFCKEVVRNANEEEYSIGSSSEESAEVATEEVKITVDDKHYQKALNEINQFYQKFPQYLQYLYQGPIVLNPWDQVKRNAVPITPTLNREQLSTSEENSKKTVDMESTEVFTKKTKLTEEEKNRLNFLKKISQRYQKFALPQYLKTVYQHQKAMKPWIQPKTKVIPYVRYL |
| 预测分子量 | 26,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSN1S2(α-s1酪蛋白亚型)重组蛋白的3篇文献示例及其摘要概括(注：以下文献为模拟内容，实际文献需通过学术数据库检索)：
1. **文献名称**: "Expression and purification of recombinant CSN1S2 in Escherichia coli for immunological studies"
**作者**: Zhang L, et al.
**摘要**: 本研究成功构建了CSN1S2基因的重组表达载体，并利用大肠杆菌表达系统实现高效可溶性表达。通过亲和层析纯化获得高纯度蛋白，并验证其在乳蛋白过敏原特异性IgE检测中的应用潜力。
2. **文献名称**: "Functional characterization of recombinant CSN1S2 in mammary epithelial cell lines"
**作者**: Gupta R, et al.
**摘要**: 通过哺乳动物细胞表达系统(如CHO细胞)重组生产CSN1S2蛋白，分析其磷酸化修饰对乳蛋白胶束形成的影响，揭示了该蛋白在乳汁分泌过程中的结构功能关系。
3. **文献名称**: "Comparative analysis of CSN1S2 allelic variants using recombinant protein technology"
**作者**: Wang Y, et al.
**摘要**: 利用重组蛋白技术比较不同物种(牛、山羊、人)CSN1S2的遗传变异，发现特定氨基酸位点差异可能影响蛋白消化特性及致敏性，为低过敏乳制品开发提供依据。
4. **文献名称**: "CSN1S2-derived bioactive peptides: Recombinant production and bioactivity screening"
**作者**: Martínez MJ, et al.
**摘要**: 通过重组表达CSN1S2并酶解生成多肽库，筛选出具有抗菌和抗氧化活性的短肽，证明其在功能性食品或药物开发中的潜在价值。
(提示：实际文献需通过PubMed/Google Scholar以关键词“CSN1S2 recombinant”、“alpha-s1-casein expression”等检索，并优先选择近5年发表的蛋白表达或应用相关研究。)
CSN1S2. also known as alpha-s2 casein, is a major milk protein component in ruminants, particularly cows. As a member of the casein family, it plays a critical role in nutrient delivery and micelle formation during milk secretion. This phosphorylated glycoprotein contains multiple phosphorylation sites and disulfide bonds, contributing to its structural complexity. Naturally occurring CSN1S2 exists as a genetic variant with polymorphisms influencing milk quality traits.
Recombinant CSN1S2 production emerged to overcome challenges in studying native caseins, which are difficult to purify from milk due to similar physicochemical properties among casein subtypes. Through genetic engineering, the CSN1S2 gene is cloned into expression vectors and expressed in heterologous systems like E. coli or mammalian cells. However, achieving proper post-translational modifications (e.g., phosphorylation) often requires eukaryotic expression systems.
The recombinant protein enables precise investigation of CSN1S2's biological functions, including its interaction with calcium phosphate in micelle stabilization and its digestibility patterns. Researchers utilize it to study milk allergy mechanisms, as CSN1S2 contains IgE-binding epitopes relevant to cow's milk protein allergy. In biotechnology, it serves as a model for developing casein-based biomaterials and drug delivery systems due to caseins' self-assembly properties.
Recent applications extend to nutritional science, where recombinant CSN1S2 helps evaluate genetic variants' impact on milk processing characteristics and digestibility. Its controlled production also supports standardization in immunological studies and diagnostic reagent development. Ongoing research focuses on optimizing expression yields while maintaining native-like structural features critical for functional studies.
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